Association of single nucleotide polymorphism rs2065955 of the filaggrin gene with susceptibility to Epstein-Barr virus-associated gastric carcinoma and EBV-negative gastric carcinoma  

作　　者：Xiaojing Kuang Lingling Sun Shuzhen Liu Zhenzhen Zhao Danrui Zhao Song Liu Bing Luo 

机构地区：[1]Department of Medical Microbiology,Qingdao University Medical College [2]Department of Pathology,The Affiliated Hospital of Qingdao University [3]Department of Blood Transfusion,The Affiliated Hospital of Qingdao University

出　　处：《Virologica Sinica》2016年第4期306-313,共8页中国病毒学（英文版）

基　　金：funded by the National Natural Science Foundation of China (NSFC 81571995);Specialized Research Fund for the Doctoral Program of Higher Education,(20133706110001);the Natural Science Foundation of Shandong Province (ZR2015HM069)

摘　　要：The relationship between the Filaggrin gene(FLG) rs2065955 polymorphism and susceptibility to Epstein-Barr virus(EBV)-associated gastric carcinoma(EBVa GC) and EBV-negative gastric carcinoma(EBVn GC) was investigated in Shandong Province,China.We detected the FLG rs2065955 genotype and allele distribution by using PCR and restriction fragment length polymorphism(RFLP) in 64 EBVa GC,82 EBVn GC,and 111 normal control samples.Immunohistochemistry was used to detect the level of FLG protein in 35 EBVa GC and 51 EBVn GC tumor tissues.Compared with normal controls,the genotype CC and allele C of FLG rs2065955 showed higher frequency in EBVa GC and EBVn GC.There was no significant difference between EBVa GC and EBVn GC in allele distribution of FLG rs2065955,but the genotype CC was found more frequently in EBVa GC than in EBVn GC.The risk of developing either EBVa GC or EBVn GC in genotype CC was higher than in other genotypes.Furthermore,genotype CC of FLG rs2065955 may contribute more to the risk of developing EBVa GC than EBVn GC.There was no significant difference in the expression level of FLG protein between EBVa GC and EBVn GC.In conclusion,the FLG rs2065955 polymorphism was significantly related to gastric carcinoma.Allele C of FLG rs2065955 could be a risk factor for EBVa GC or EBVn GC,while genotype CC of FLG rs2065955 was especially associated with EBVa GC.The relationship between the Filaggrin gene （FLG） rs2065955 polymorphism and susceptibility to Epstein-Barr virus （EBV）-associated gastric carcinoma （EBVaGC） and EBV-negative gastric carcinoma （EBVnGC） was investigated in Shandong Province, China. We detected the FLG rs2065955 genotype and allele distribution by using PCR and restriction fragment length polymorphism （RFLP） in 64 EBVaGC, 82 EBVnGC, and 111 normal control samples. Immunohistochemistry was used to detect the level of FLG protein in 35 EBVaGC and 51 EBVnGC tumor tissues. Compared with normal controls, the genotype CC and allele C of FLG rs2065955 showed higher frequency in EBVaGC and EBVnGC. There was no significant difference between EBVaGC and EBVnGC in allele distribution of FLG rs2065955, but the genotype CC was found more frequently in EBVaGC than in EBVnGC. The risk of developing either EBVaGC or EBVnGC in genotype CC was higher than in other genotypes. Furthermore, genotype CC of FLG rs2065955 may contribute more to the risk of developing EBVaGC than EBVnGC. There was no significant difference in the expression level of FLG protein between EBVaGC and EBVnGC. In conclusion, the FLG rs2065955 polymorphism was significantly related to gastric carcinoma. Allele C of FLG rs2065955 could be a risk factor for EBVaGC or EBVnGC, while genotype CC of FLG rs2065955 was especially associated with EBVaGC.

关 键 词：Filaggrin gene(FLG) rs2065955 gastric carcinoma Epstein-Barr virus(EBV) gene polymorphism immunohistochemistry 

分 类 号：R735.2[医药卫生—肿瘤]