粘性丝孢酵母β-葡萄糖苷酶基因部分CDs区克隆与表达分析  

Cloning and expression analysis of the partial CDs of β-glucosidase gene in Trichosporon mucoides

作　　者：代军飞 牛锋[2] 钟琦[1] 吴慧昊[2] 

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730030 [2]西北民族大学实验中心,甘肃兰州730030

出　　处：《西北民族大学学报（自然科学版）》2016年第1期33-37,共5页Journal of Northwest Minzu University(Natural Science)

基　　金：降脂菌的筛选鉴定和开发利用(XBMU-2011-BC-40)

摘　　要：以粘性丝孢酵母BG基因为研究对象,采用PCR方法克隆出BG基因的部分CDs区片段,并用RT-PCR方法分析了BG在不同培养条件下的表达量.实验结果如下:克隆扩增获得长为570 bp,编码189个氨基酸的部分CDs区片段.通过实时荧光定量PCR方法分析粘性丝孢酵母BG基因在不同pH、C/N、温度以及培养时间下的表达量.结果显示培养温度、pH、C/N对粘性丝孢酵母BG基因表达量无显著性影响.培养时间对其具有显著性影响,呈先上升后下降的趋势,在第3 d时表达量最高且极显著高于其他培养时期.此研究为进一步研究该基因的功能奠定了基础.BG（ffglucosidase） of Trichosporon mucoides was used as research subject., The partial CDs of BG gene was cloned by polymerase chain reaction （PCR） from Trichosporon mucoides. BG mRNA gene was examined in different culture conditions by RT-PCR. Results showed that the partial CDs of BG gene was 570bp, which encoded 189 amino acids. BG gene was analyzed in different pH, carbon and nitrogen ratio（C/N）, temperature and time by RT-PCR. Temperature, pH and C/N had no significant effect on the expression of BG gene in Trichosporon mucoides, whereas time was differ- ent from them. The expression level in 3d was significantly higher than that in other culture phase, and was decreased after increasing. This finding may provide basic data for further studying the role of ^-glucosidase gene in Trichosporon rnucoides.

关 键 词：粘性丝孢酵母 Β-葡萄糖苷酶 CDs克隆 表达分析 

分 类 号：Q786[生物学—分子生物学]