碳源对米曲霉M-4共代谢降解3-苯氧基苯甲酸的影响  

Effects of carbon sources on 3-phenoxybenzoic acid degradation by Aspergillus oryzae M-4 in co-metabolism way

作　　者：苏宏南[1] 喻志强[1] 黄紫霖 迟原龙[1] 贾冬英[1] 姚开[1] 

机构地区：[1]四川大学轻纺与食品学院,成都610065 [2]成都大学医护学院,成都610106

出　　处：《食品科技》2016年第8期19-23,共5页Food Science and Technology

基　　金：国家自然科学基金项目(31371775)

摘　　要：以通过共代谢方式对3-苯氧基苯甲酸具有较强生物降解能力的米曲霉M-4为实验菌株,考察了碳源对该菌株降解3-苯氧基苯甲酸的影响,分析了该菌株降解3-苯氧基苯甲酸的动力学特征。结果表明,淀粉、葡萄糖、果糖、核糖和琥珀酸均利于共代谢过程中米曲霉M-4的生长;当适宜碳源葡萄糖的添加量为5.0 g/L时,该菌株在4 d时可完全降解含量为100μg/mL的3-苯氧基苯甲酸,其降解速率常数(k)和半衰期(T_(1/2))分别为6.13 d^(-1)和2.32 d。In this study, Aspergillus oryzae M-4 with high biodegradation capacity on 3-phenoxybenzoic acid was used. Effects of carbon sources on 3-phenoxybenzoic acid degradation by the strain in cometabolism way were investigated, and the kinetics of 3-phenoxybenzoic acid degradation was analyzed. The results showed that starch, glucose, fructose, ribose and succinic acid all accelerated the growth of the strain in the process of co-metabolism, of which glucose was its suitable carbon source. At 5.0 g/L of glucose, the strain could completely remove 100 μg/mL of 3-phenoxybenzoic acid on the fourth day, and the rate constant（k） and half-life（T1/2） of 3-phenoxybenzoic acid degradation were 6.13 d^-1 and 2.32 d, respectively.

关 键 词：3-苯氧基苯甲酸 生物降解 米曲霉M-4 碳源 共代谢 

分 类 号：TS201.3[轻工技术与工程—食品科学]