固相萃取-高效液相色谱荧光法测定水果中1-萘乙酸残留量  

Determination of 1-naphthylacetic acid in fruits by solid phase extraction and high performance liquid chromatography with fluorescence detector

作　　者：仲伶俐[1] 胡莉[1] 付成平[1] 郭灵安[1] 毛建霏[1] 李曦[1] 叶先林[1] 

机构地区：[1]四川省农业科学院分析测试中心,农业部农产品质量安全风险评估实验室(成都),成都610066

出　　处：《食品科技》2016年第8期289-292,共4页Food Science and Technology

基　　金：2015年国家农产品质量安全风险评估项目(GJFP2015001);成都市科技局高校院所应用成果转化项目(12DXYB284NC-005)

摘　　要：建立了固相萃取-高效液相色谱荧光法测定水果中1-萘乙酸（NAA）残留量的方法。样品经乙腈提取,氨基固相萃取小柱净化,用高效液相色谱荧光检测器测定。经过验证,方法定量限为0.002 mg/kg,当水果中添加NAA水平为0.004、0.04、0.4 mg/kg时,回收率为82.1%-114.1%之间,相对标准偏差（RSD）为0.6%-10.5%。该方法准确度好、灵敏度高,适合水果中NAA残留量的测定。A method for determination of 1-naphthylacetic acid（NAA） in fruits by solid phase extraction and high performance liquid chromatography with fluorescence detector was developed. The sample was extracted with acetonitrile and purified by NH_2 SPE cartridges. The analysis were determined by high performance liquid chromatography with fluorescence detector. The limit of quantification（LOQ） of the developed method was 0.002 mg/kg. Recoveries of NAA ranged from 82.1% to 114.1% when the spiked levels of 0.004, 0.04, 0.4 mg/kg, with the relative standard deviations（RSDs） were 0.6%～10.5%. The method is sensitive and accurate for determination of NAA in fruit.

关 键 词：固相萃取 高效液相色谱 荧光法 1-萘乙酸 

分 类 号：TS207.3[轻工技术与工程—食品科学]