氧嗪酸钾联合酵母对SD大鼠尿酸生成和排泄及肾功能的影响  

Effect of yeast and potassium oxonate on formation and excretion of uric acid and renal function of SD rats

作　　者：聂安政 林志健[1] 王雨[1] 周月[1] 孙小霞[1] 李凡[1] 蔡萌[1] 周伟龙[1] 张冰[1] 

机构地区：[1]北京中医药大学中药学院临床中药系,北京100029

出　　处：《中国药理学与毒理学杂志》2016年第8期855-859,共5页Chinese Journal of Pharmacology and Toxicology

基　　金：高等学校博士学科点专项科研基金课题(20120013130002);高等学校博士学科点专项科研基金课题(20130013120001);国家自然科学基金(81403152)~~

摘　　要：目的观察氧嗪酸钾联合酵母对大鼠血尿酸(UA)水平及UA生成、排泄和肾功能的影响,探讨氧嗪酸钾联合酵母诱导大鼠高尿酸血症的作用特点。方法 SD大鼠分别给予酵母膏21 g·kg^(-1)+氧嗪酸钾100,200和300 mg·kg^(-1),每日1次,连续35 d。分别于第14,28和35天检测血清UA、肌酐(CRE)和尿素氮(BUN)水平及腺苷脱氨酶(ADA)、黄嘌呤氧化酶(XOD)和鸟嘌呤脱氨酶(Gu Da)活性,第28天检测尿液UA、CRE、BUN和尿蛋白水平与尿比重、尿量并计算UA排泄量和UA清除率,第35天处死大鼠取右肾称重并计算肾指数。结果与正常组比较,第14,28和35天3个给药组血UA水平均显著升高(P<0.01);第35天3个给药组血CRE水平均显著升高(P<0.05,P<0.01);第14天酵母膏21 g·kg^(-1)+氧嗪酸钾100和300 mg·kg^(-1)组ADA水平显著降低(P<0.05),第14和28天酵母膏21 g·kg^(-1)+氧嗪酸钾200 mg·kg^(-1)组ADA活性水平显著降低(P<0.01);第14和28天酵母膏21 g·kg^(-1)+氧嗪酸钾300 mg·kg^(-1)组XOD活性显著升高(P<0.05,P<0.01);第28天3个给药组UA排泄量和UA清除率显著降低(P<0.05,P<0.01);第35天3个给药组肾指数显著升高(P<0.01)。结论酵母膏联合氧嗪酸钾使大鼠血清UA水平升高,可能与XOD和ADA活性变化及肾UA排泄障碍有关,UA排泄障碍的发生可能与肾功能的变化有关。OBJECTIVE To investigate the effect of potassium oxonate and yeast on formation and excretion of uric acid （UA） and renal function in rats, and to describe the characteristics of hyper- uricemia in rats induced by potassium oxonate and yeast. METHODS SD rats were given yeast 21 g·kg-1 and potassium oxonate 100,200 and 300 mg·kg-1 , respectively, once a day for 35 d. Levels of UA, creatinine （CRE） , urea nitrogen （BUN） and the activity of adenosine deaminase （ADA） , xanthine oxidase （XOD）, guanine deaminase （GuDa） in serum were determined on the 14th, 28th and 35th day, respectively. Levels of UA, CRE, BUN, protein in urine, urine specific gravity and urine volume were determined on the 28th day while UA excretion and clearance were counted. These rats were sacrificed on the 35th day to weigh the right kidney and calculate the kidney index. RESULTS Compared with normal control group, serum UA levels of three treatment groups were significantly higher from the 14th to the 35th day（P〈0.01 ） ; CRE levels of three treatment groups were significantly higher on the 35th day（P〈0.05, P〈0.01） ; ADA activity was significantly lower in the groups of yeast 21 g. kg-1 and potassium oxonate 100 and 300 mg-kg-1 on the 14th day （P〈0.01） and in the group of yeast 21 g. kg-1 and potassium oxonate 200 mg. kg-1 on the 14th to 28th day（P〈0.05） ; XOD activity of the groups of yeast 21 go kg-1 and potassium oxonate 300 mg. kg-1 was significantly higher on the 14th to 28th day（P〈0.05, P〈0.01 ） ; UA excretion and clearance of three treatment groups were significantly lower on the 28th day（P〈0.05, P〈0.01 ） ; the kidney index of the three treatment groups was significantly higher on the 35th day（P〈0.01）. CONCLUSION Yeast and potassium oxonate can elevate levels of UA in rats, which may be related to the changes of XOD and ADA activity and the disturbance of UA excretion that is likely associated with kidney damage.

关 键 词：酵母 氧嗪酸钾 血尿酸 尿酸排泄 肾功能 

分 类 号：R965[医药卫生—药理学]