快速检测生鲜肉中三种食源性致病菌  

Rapid detection of three foodborne pathogens in raw meat

作　　者：张帅[1] 齐颖颖[1] 张红星[1] 王怡雯[1] 谢远红[1] 刘慧[1] 

机构地区：[1]北京农学院食品科学与工程学院/食品质量与安全北京实验室/农产品有害微生物及农残安全检测与控制北京市重点实验室/北京市食品安全免疫快速检测工程技术研究中心/微生态制剂关键技术开发北京市工程实验室,北京102206

出　　处：《江苏农业学报》2016年第4期939-945,共7页Jiangsu Journal of Agricultural Sciences

基　　金：北京市属高等学校高层次人才引进与培养长城学者计划(CIT&TCD20140315);北京农学院协同创新建设项目

摘　　要：为同时快速检测生鲜肉中鼠伤寒沙门氏菌、单增李斯特菌和福氏志贺氏菌3种食源性致病菌,降低检测成本,制备了特异性单克隆抗体和多克隆抗体,研制出能够同时检测以上3种致病菌的胶体金免疫层析试纸条。结果表明,本试验成功筛选出3株稳定分泌抗体的杂交瘤细胞株2E3、2E7和2E8,3种单克隆抗体效价均在128×10^6以上,3种多克隆抗体效价均在200×10^6以上。制备的胶体金免疫层析试纸条对鼠伤寒沙门氏菌、单增李斯特菌和福氏志贺氏菌3种食源性致病菌的特异性良好,灵敏度均为1×10^4CFU/g。To achieve the simutaneous detection of Salmonella typhimurium, Listeria monocytogenes and Shigella flexneri in raw meat,three hybridoma cell strains, 2 E 3,2 E 7 a nd 2 E 8 , stably secreting antibodies against above pathogens were screened. The titers of three monoclonal antibodies and three polyclonal antibodies were more than 1. 28×10^6 and 2. 00×10^6,respectively. The immunochromatographic strip developed could detect all three pathogens as low as 1×10^4 CFU/g. L. monocytogenes was detected by the strip in market sample which was free of other two pathogens.

关 键 词：食源性致病菌 单克隆抗体 多克隆抗体 胶体金免疫层析试纸条 

分 类 号：S851.347.02[农业科学—预防兽医学]