β-榄香烯对兔VX2喉癌生长及肿瘤相关因子的影响  

The effects of β-elemene on rabbit VX2 laryngeal carcinoma and factors associated with tumor growth and metastasis

作　　者：赵婉君[1] 赵振安 王青云[1] 李小波[1] 谢辰[1] 曹忠胜[1] 

机构地区：[1]苏州大学附属第二医院耳鼻咽喉科,215000 [2]山东省淄博市中心医院耳鼻咽喉科,255020

出　　处：《中华耳鼻咽喉头颈外科杂志》2016年第8期606-610,共5页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

摘　　要：目的 建立喉癌动物模型以探讨β-榄香烯对兔VX2喉癌生长的抑制作用,并初步探究其作用机制.方法 新西兰大白兔66只,接种VX2肿瘤组织块悬液建立喉癌兔动物模型,随机数字表法分为对照组、顺铂组和β-榄香烯组,给予相应药物干预后处死动物,测量肿瘤体积,计算抑瘤率;免疫组化染色检测肿瘤组织增殖细胞核抗原（proliferation cell nuclear antigen,PCNA）、B淋巴细胞瘤-2（B-cell lymphoma-2,Bcl-2）、血管内皮生长因子D（VEGF-D）表达水平.结果 β-榄香烯组和顺铂组的抑瘤率分别为48.5％和51.4％;β-榄香烯组和顺铂组Bcl-2表达差异无统计学意义,但均低于对照组（P值均＜0.05）;与对照组相比,β-榄香烯组PCNA表达明显少（P ＜0.05）;β-榄香烯组VEGF-D表达水平均低于对照组和顺铂组（P值均＜0.05）.结论 β-榄香烯对兔VX2喉肿瘤的生长有明显抑制作用,其机制可能与抑制PCNA、VEGF-D和Bcl-2表达有关.Objective To investigate the inhibition effect of β-elemene on the growth and metastasis of laryngeal carcinoma and the underlying mechanism. Methods Sixty-six rabbits were vaccinated with suspension of VX2 cancer tissues to establish a rabbit laryngeal carcinoma model and then they were randomly treated with the injection of 0. 9% sodium chloride solution （as a control）, cisplatin, or β- elemene from the seventh day after vaccination. The rabbits were sacrificed three weeks after vaccination. The laryngeal tumor was dissected, and the volume of tumor and the inhibitory rate of tumor growth were measured. The expressions of PCNA （proliferation cell nuclear antigen）, Bcl-2 （B-cell lymphoma-2） and VEGF-D （vascular endothelial growth factor D ） in tumor were semiquantitatively examined by immunohistochemistry. Results The inhibition rates of tumor growth in β-elemene-treated group and the cisplatin-treated group were 48.5% and 51.4% , respectively. The expressions of Bel-2 in β-elemene-treated group and cisplatin-treated group were significantly lower than the control （ P 〈 0. 05 ）, but with no significant difference between β-elemene-treated group and cisplatin-treated group, The expression of PCNA in β-elemene-treated group was significantly decreased compared to control group （P 〈 0. 05 ）. The expression of VEGF-D in β-elemene-treated group was lower than that in control group or the cisplatin- treated group （P 〈 0. 05 ）. Conclusion β-elemene can inhibit the growth of rabbit VX2 laryngeal tumor, which may be associated with the inhibition of factors related to tumor growth and metastasis.

关 键 词：姜黄属 喉肿瘤 增殖细胞核抗原 B淋巴细胞瘤-2 

分 类 号：R739.65[医药卫生—肿瘤]