CaSR在大鼠血管收缩/舒张反应性调节中的作用  

Effects of Ca SR on vascular relaxation/constriction in rats

作　　者：彭小勇[1] 李涛[1] 刘良明[1] 杨光明[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所第二研究室创伤烧伤与复合伤国家重点实验室,重庆400042

出　　处：《局解手术学杂志》2016年第9期629-632,共4页Journal of Regional Anatomy and Operative Surgery

基　　金：国家自然科学基金(81571886);军队医学科技青年培育项目(13QNP118);教育部留学回国人员科研启动基金(2014)

摘　　要：目的研究钙敏感性受体(Ca SR)激动剂对大鼠肠系膜动脉收缩和舒张反应性的影响、以及内皮在其中的作用。方法采用内皮完整和内皮去除的大鼠肠系膜动脉,观察Ca SR特异性激动剂cinacalcet对血管基础张力和预收缩的血管张力的影响、以及不同剂量cinacalcet预处理对去甲肾上腺素(NE)诱导的血管收缩反应性和乙酰胆碱诱导的舒张反应性的影响。结果Ca SR激动剂cinacalcet对静息状态下内皮完整和内皮去除的血管基础张力均无明显影响。在内皮完整的血管环,cinacalcet对预收缩血管有明显的舒张作用,并呈剂量依赖性;内皮去除取消了cinacalcet的舒张作用。Cinacalcet预处理30 min,使内皮完整的血管的收缩反应性明显降低,而对舒张反应性无明显影响。对去除内皮的血管环,cinacalcet预处理对血管收缩/舒张反应性均无明显影响。结论 Ca SR在血管收缩反应性调节中有重要作用,并有明显的内皮依赖性。Objective To investigate the effects of calcium sensing receptor （CaSR）on vasorelaxation/vasoconstriction of superior mes-enteric artery （SMA）in rats and its relationship to endothelium.Methods With endothelium-intact and endothelium-denuded SMA rings of rats,the effects of CaSR-specific allosteric modulator cinacalcet on the SMA rings pre-contracted with norepinephrine （NE）,and vascular contractile response /relaxation reactivity were observed.Results Cinacalcet had no effects on resting tension of SMA rings with or without endothelium.Cinacalcet caused a significant relaxation in the endothelium-intact SMA rings pre-contraction with NE in a dose-dependent manner.Endothelium denudation abolished cinacalcet-induced vasorelaxation.Pretreatment with cinacalcet for 30 minutes decreased the con-tractile response of endothelium-intact SMA rings to NE,but had no significant influence on relaxation reactivity.In the endothelium-denuded SMA rings,cinacalcet did not affect both vasoconstriction and vasorelaxation.Conclusion CaSR plays an important role in the regulation of the vascular reactivity,and this effect is endothelium-dependent.

关 键 词：钙敏感性受体 CINACALCET 内皮 收缩反应性 舒张反应性 

分 类 号：R392[医药卫生—免疫学]