中医“同病异证”理论在老年期痴呆两个典型证候的Tau蛋白脯氨酸富集区磷酸化位点的体现  

Reflect on Tau protein in proline-rich region of phosphorylation sites of two typical syndromes with senile dementia disease based on the same disease with different syndromes of Chinese medicine theory

作　　者：郭斌[1] 郭蕾[1] 韩诚[1] 师卫斌 

机构地区：[1]山西中医学院,山西太原030024

出　　处：《山西中医学院学报》2016年第3期17-19,22,共4页Journal of Shanxi College of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81173165)

摘　　要：目的:观察老年期痴呆病证结合肾虚精亏证大鼠模型与痰浊阻窍大鼠模型Tau蛋白在脯氨酸富集区磷酸化位点的情况,分析磷酸化的特异性。方法:采用隔周结扎双侧颈总动脉及切断术,随后腹腔注射D-半乳糖,建立老年期痴呆病大鼠模型;随后在疾病模型的基础上分别进行房劳水劳刺激和投喂高脂乳剂的刺激,建立相应的病证结合老年期痴呆大鼠模型。最后采用免疫印迹方法观察Tau蛋白在脯氨酸富集区Thr181、Thr205、Thr231、Ser262磷酸化位点的异常磷酸化状况。结果:在Tau蛋白脯氨酸富集区,与老年期疾呆组相比,病证结合痰浊阻窍证组主要在205和231位磷酸化水平显著增强,病证结合肾虚精亏证组主要在181,205,231位磷酸化水平显著增强。结论:老年期痴呆各典型证候在Tau蛋白脯氨酸富集区的磷酸化位点上有特异性,揭示了"同病异证"理论的科学内涵。Objective ：To observe Tau protein in proline-rich region of phosphorylation sites of two typical syndromes with senile dementia disease rat model and to analyze its specific phosphorylation of two syndromes, reflecting the same disease with different syndromes＇of Chinese medicine theory. Methods ：Two carotid arteries were ligated one week apart, Dgalactose was injected in intraperitoneal to establish Senile dementia rats model,subjected to fatigue of working and sexy stimuli and raise fat diet milk in the model on the basis of disease,then the corresponding rat model of senile dementia was established,which combined of disease and syndrome. Followed by Western blot method,to observed abnormal phosphorylation of Tan protein in the proline-rieh region Thrl81 ,Thr205,Thr231, Ser262 phosphorylation sites. Result：In the Tau protein proline-rich region,compared with the Senile Dementia group,the disease with phlegm blocking orifices syndrome group significantly phosphorylation levels were increased at 205 and 231 sites,phosphorylation levels of the disease with kidney and essence deficiency syndrome group were significantly increased in 181,205,231 sites. Conclusion： All typical senile dementia syndromes have specific phosphorylation sites on Tan protein proline-rich region, revealing the scientific connotation of “the same disease with different svndrome”theory.

关 键 词：老年期痴呆 证候 病证结合 Tau蛋白磷酸化位点 

分 类 号：R228[医药卫生—中医基础理论]