沉默人宫颈癌细胞Hela中FAL1的表达对人宫颈癌细胞Hela增殖、侵袭及迁移的影响  

Effect of silencing FAL1 expression on proliferation, invasion and migration of human cervical carcinoma Hela cells

作　　者：石兴源[1] 余宏伟[1] 黄文瑾[1] 廖志伟[1] 贾保昌[1] 罗凯[2] 

机构地区：[1]广州医科大学附属肿瘤医院,广东广州510095 [2]广州医科大学肿瘤研究所,广东广州510095

出　　处：《中国现代医学杂志》2016年第17期34-38,共5页China Journal of Modern Medicine

基　　金：广州医科大学青年基金(No:2013A45)

摘　　要：目的探讨沉默人宫颈癌细胞Hela中功能基因组趋化基因(FAL1)的表达对人宫颈癌细胞Hela增殖、侵袭及迁移的影响。方法设计并合成FAL1的si RNA片段转染Hele细胞分别命名为FAL1-si RNA/Hela细胞组和FAL1-NC/Hela细胞组。比较两组Hela细胞转染FAL1-si RNA后的FAL1 m RNA相对表达量、光密度(OD)值、穿膜细胞数、迁移距离的差异。结果 FAL1-si RNA/Hela细胞的FAL1 m RNA相对表达量(0.49±0.21)低于FAL1-NC/Hela细胞(1.34±0.13),差异有统计学意义(P<0.05)。FAL1-si RNA/Hela细胞在72、96和120 h的OD值分别为(2.04±0.15)、(2.43±0.08)和(2.48±0.15),低于FAL1-NC/Hela细胞的(2.36±0.18)、(3.11±0.27)和(3.44±0.26),差异有统计学意义(P<0.05);FAL1-si RNA/Hela细胞的穿膜细胞数(126.18±8.75)个低于FAL1-NC/Hela细胞(208.54±6.75)个,差异统计学意义(P<0.05);FAL1-si RNA/Hela细胞在24、48和72 h的细胞迁移距离为(6.34±1.48)、(11.65±2.52)和(15.08±3.19)μm低于FAL1-NC/Hela细胞的(11.42±2.73)、(19.56±4.33)和(27.71±5.12)μm,差异有统计学意义(P<0.05)。结论 FAL1-si RNA/Hela细胞的FAL1m RNA相对表达量下降,进而抑制Hela细胞的增殖、侵袭和迁移能力。Objective To study the effects of silencing FALl expression on the proliferation, invasion and migration of the human cervical carcinoma Hela cells. Methods SiRNA FALl fragment was designed, synthesized and transfeeted into Hela cells, which were named FALI-siRNA/Hela cell group and FAL1-NC/ Hela cell group. The differences in FALl mRNA relative expression, OD value, number of transmembrane cells and migration distance were compared between the two groups after transfeetion. Results The relative expression of FALl mRNA in the FALI-siRNA/Hela cells （0.49 ±0.21） was significantly lower than that of FAL1-NC/Hela cells [（1.34± 0.13）, P〈 0.05]. The OD value of the FALI-siRNA/Hela cells in 72, 96 and 120 h [（2.04 ± 0.15）, （2.43 ± 0.08） and （2.48 ±0.15） respectively] was significantly lower than that of the FALI-NC/Hela cells [（2.36 ±0.18）, （3.11 ± 0.27） and （3.44 ± 0.26） respectively, P〈 0.05]. The number of transmembrane cells in the FALI-siRNA/Hela cells （126.18±8.75） was significantly smaller than that of the FAL1-NC/Hela cells [（208.54 ± 6.75）, P〈 0.05]. The cell migration distance of the FALI-siRNA/Hela ceils in24, 48 and 72h [（6.34±1.48）, （11.65± 2.52） and （15.08± 3.19） p,m respectively] was significantly shorther than that of the FAL1-NC/Hela cells [（11.42 ± 2.73）, （19.56±4.33） and （27.71 ± 5.12） p,m respectively, P〈 0.05]. Conclusions The relative expression of FALl mRNA in the FALI-siRNA/Hela cells decreases, which inhibits proliferation, invasion and migration of Hela cells.

关 键 词：宫颈癌 HELA细胞 功能基因组趋化基因siRNA 增殖 侵袭 迁移 

分 类 号：R737.33[医药卫生—肿瘤]