NJ001识别抗原在多种肿瘤组织中的表达及其与肿瘤分化的关系  

Expression of NJ001 recognized antigen and its association with tumor differentiation in multiple neoplasms

作　　者：荆俊鹏 王悦 吴蕾 余汉中 蒋欣华 李月梅 王芳[1] 徐建 顾春荣 孙瑞红 潘世扬 

机构地区：[1]南京医科大学第一附属医院检验学部,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2016年第7期826-829,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81371894);江苏省实验诊断学重点实验室(XK201114);江苏高校优势学科建设工程基金项目;教育部博导基金(20113234110012);国家临床重点专科建设项目

摘　　要：目的 ：研究NJ001识别抗原在人体内多种恶性肿瘤组织中的表达情况,并评估其与肿瘤分化的关系。方法 ：采用免疫组化的方法对肺腺癌、肺鳞癌、胰腺癌、乳腺癌、食管癌、胃癌、肝癌和对应的良性病变组织中NJ001识别抗原的表达进行检测,同时与肿瘤分化程度进行比较,通过半定量的方法判定结果,使用χ~2检验或Fisher确切概率法进行相关性分析。结果：NJ001识别抗原在肺腺癌（88.0%）、肺鳞癌（82.2%）、胰腺癌（73.3%）和乳腺癌（57.8%）中均呈现出高表达,并且均与肿瘤分化程度显著相关（肺腺癌P〈0.001,肺鳞癌P=0.002,胰腺癌P=0.002,乳腺癌P=0.024）。结论 ：NJ001识别抗原有望为临床诊断肺腺癌、肺鳞癌、胰腺癌和乳腺癌及判断其分化程度提供一个新的肿瘤标志物。Objective：To examine N J001 recognized antigen expression in surgically resected human malignant tumor tissues,and to evaluate the relationship between its expression and tumor differentiation. Methods：NJ001 recognized antigen expression was immunohistoehemically studied in lung adenocarcinoma,lung squamous carcinoma,pancreatic cancer,breast cancer, esophageal cancer, gastric cancer, liver cancer and matched non-neoplastic tissues, and all positive cases were compared on tumor differentiation. The results were scored by semiquantitative analysis,and statistical analysis was evaluated by chi-square test or Fisher exact test. Results：Expression of NJ001 recognized antigen was significantly high in lung adenoearcinoma （88.0%）,lung squamous carcinoma （82.2%）, pancreatic eaneer（73.3%）and breast cancer（57.8%）, and was significantly correlated with lower differentiation degree in all these tumors（P 〈 0.001 in lung adenocarcinoma;P=0.002 in lung squamous carcinoma;P=0.002 in pancreatic cancer;and P=0.024 in breast cancer）. Conclusion：NJ001 recognized antigen is expected to be a tumor marker in lung adenocarcinoma,lung squamous carcinoma,breast cancer and pancreatic cancer,as well as a remarkable biomarker for identification of differentiation degree in multiple neoplasms.

关 键 词：单克隆抗体 肿瘤标志物 肿瘤 分化 免疫组化 

分 类 号：R730.3[医药卫生—肿瘤]