电针合谷穴对窒息大鼠心肺复苏后神经功能及生存率的影响  

Effect of electroacupuncture at Hegu acupoints on survival and neurological function after cardiopulmonary resuscitation in asphyxial cardiac arrest rats

作　　者：房巍[1] 梁小燕[1] 庄永敬[2] 曹云飞[1] 周黎明[1] 

机构地区：[1]解放军421医院急诊科,广州510318 [2]解放军421医院普通烧伤外科,广州510318

出　　处：《中华灾害救援医学》2016年第9期481-485,共5页Chinese Journal of Disaster Medicine

基　　金：广东省中医药局科研课题(20151039);全军"十二五"医学创新专项课题(13CXZ045);2014年度全军医学科技青年培育项目计划(14QNP047)

摘　　要：目的观察电针刺激合谷穴对窒息大鼠心肺复苏(cardiopulmonary resuscitation,CPR)后神经功能及生存率的影响。方法采用呼气末夹闭气管窒息法建立大鼠心脏停跳CPR模型,雄性SD大鼠随机分为四组:(1)CPR对照组(C组):右侧脑室注射生理盐水(5μl);(2)甲硝阿托品处理组(A组):右侧脑室注射甲硝阿托品(5μg/kg);(3)单纯电针刺激处理组(E组):电针刺激合谷穴+右侧脑室注射生理盐水;(4)电针刺激加甲硝阿托品处理组(EA组):电针刺激合谷穴+右侧脑室注射甲硝阿托品。其中每组8只大鼠用于神经功能观察,窒息时间5 min,分别于自主循环恢复(restoration of spontaneous circulation,ROSC)后24、48、72 h进行神经功能缺损评分(neurological deficit scores,NDS);12只大鼠用于生存率观察,窒息时间8 min,每隔2 h记录ROSC后72 h内的存活情况。结果 ROSC后24 h和48 h,四组间NDS评分整体比较,差异无统计学意义(χ2=3.828,P=0.281;χ2=3.728,P=0.292)。ROSC后72 h,四组间NDS评分整体比较,差异有统计学意义(χ2=8.710,P=0.033);进一步两两比较显示,E组NDS评分高于C组(P=0.015),差异有统计学意义。C组、E组、A组及EA组72 h生存率分别为25.00%(3/12)、66.67%(8/12)、16.67%(2/12)和33.33%(4/12)。组间比较显示:E组72 h生存率高于C组,差异有统计学意义(χ2=3.874,P=0.049)。结论电针刺激合谷穴可有效提高窒息大鼠CPR后的神经功能和生存率,其作用可能与激活中枢胆碱能系统功能有关。Objective To investigate effect of electroacupuncture at Hegu acupoints on survival rate and neurological functionafter cardiopulmonary resuscitation in asphyxia1 cardiac arrest rats. Methods Asphyxial cardiac arrest was induced with asphyxia byclamping the tracheal tubes. Male Sprague-Dawley rats were randomly divided into five groups （1） cardiopulmonary resuscitation group（group C）, with right intracerebroventricular injection of 5 μl physiologic saline; （2） atropine methyl nitrate administered group （group A）,with right intracerebroventricular injection of atropine methyl nitrate （5 μg/kg）; （3） electroacupuncture administered group （group E）, withelectroacupuncture at Hegu acupoints and right intracerebroventricular injection of physiologic saline; （4） electroacupuncture plus atropinemethyl nitrate group （group EA）, with electroacupuncture at Hegu acupoints and right intracerebroventricular injection of atropine methylnitrate. 8 rats in each group were used for neural function observation. They were subjected to 5 minutes of asphyxial cardiac arrest, andthen neurological deficits were evaluated and scored at 24, 48 and 72 h respectively after restoration of spontaneous circulation （ROSC）.12 rats in each group were used for survival study. They were also subjected to 8 minutes of asphyxial cardiac arrest, and then survivalconditions were observed every 2 h within 72 h after ROSC. Results At 24 h and 48 h after ROSC, the differences in neurological deficit scores （NDS） among all four groups was not statistically significant（χ ^2=3.828, P=0.281; χ^2=3.728, P=0.292）, but at 72 h after ROSC,NDS showed statistically significant differences among four groups.And then further pairwise comparison showed that NDS in groupE was significantly higher than that in group C, and the differencewas statiscally significant （P=0.015）. Survival rate within 72 hof group C, group E, group A and group EA were 25% （3/12）, 66.67% （8/12）, 16.67% （2/12） and 33.33% （4/

关 键 词：电针 窒息 心肺复苏 神经功能 生存率 

分 类 号：R245[医药卫生—针灸推拿学]