高效液相色谱法检测优化诃子没食子酸萃取工艺  

Assay of Gallic Acid from Terminalia chebula Retz and Optimization of Ultrasonic-assisted Extraction with High Performance Liquid Chromatography

作　　者：李忠琴[1,2,3] 林茂[1,2,3] 赖晓健 李秋云[2,3] 徐继松 江兴龙[1,2,3] 

机构地区：[1]集美大学水产学院,福建厦门361021 [2]鳗鲡现代产业技术教育部工程研究中心,福建厦门361021 [3]厦门市渔用药物工程技术研究中心,福建厦门361021

出　　处：《西华大学学报（自然科学版）》2016年第5期34-38,共5页Journal of Xihua University:Natural Science Edition

基　　金：公益性行业(农业)科研专项(201203085);海洋经济发展区域示范项目(14PYY050SF03);国家海洋局海洋经济创新发展区域示范专项(2012FJ03);福建省教育厅项目(JA15260);厦门市重点项目(3502Z20110014);鳗鲡现代产业技术教育部工程研究中心开放基金(RE201606,RE201607);大学生创新创业训练计划项目(201510390053)

摘　　要：为优化诃子的鞣质类抗菌成分的萃取工艺,建立-种定量检测诃子萃取液中没食子酸质量浓度的高 效液相色谱法,其回归方程为4=92.48C-28.566,没食子酸质量浓度在4 - 2 0 μg ·ml^-1范围与峰面积呈良好的线 性关系.所用色谱柱为 Hypersil 0DS （ 4.0 mm×250 mm,5μm ） ,流 动 相 为 F（甲醇）： F （ 0 .1%磷酸 ）= 4 ： 9 6 ,检测 波长 270 mn,流速 1.0 mL · min^-1 ,柱温 25℃.同时利用该方法作为超声辅助萃取诃子没食子酸的评价指标,采用 正交实验设计优化获得最佳萃取工艺条件为溶剂60% 乙醇,料液比 1：2 0 ,温度 50℃：,萃取时间80min.The process conditions of extracting gallic acid from Terminalia chebula Retz. was optimized with ultrasonic - assisted extraction. High performance liquid chromatography（ HPLC） was applied to assay gallic acid extracted from Terminalia chebula Retz. At the same time this method was applied as indexes to evaluate the yields of extracting gallic acid from Terminalia chebula Retz. The orthogonal experiment was applied to optimize the conditions of ultrasonic - assisted extraction for extracting gallic acid from T e rm in a l ia chebula Retz. The conditions which were ethanol concentration, solid - liquid ratio, ultrasonic time and ultrasonic temperature, were investigated on the process of extracting gallic acid. The regression linear equation is A =92.48 × C -28.566, which has good linear relationship with the peak area among 4 （ μg·mL^ -1 ） to 20 （ μg·mL^ -1 ） . The assaying condition is that column is Hypersil ODS （4. 0 mm x 250 mm,5 jjim）,mobile phase is 4% methanol to 96% water with 0. 1% phosphoric acid （ F- F） , and detection wave-length is 270 nm, and flow rate 1.0 （ mL · min ^-1 ） , column temperature 25 . The optimized conditions of ultrasonic - assisted ex-traction, solvent are 60% ethanol （ V7- F） , ratio of material to liquid 1 ： 20, temperature of solvent 50℃ , extraction duration 80 min. The experiment results show that this quantitative detection method is accurate, reliable and precise for assaying gallic acid with HPLC, and the application to evaluating the best conditions of extracting gallic acid from Terminalia chebula is successful. The process of u lt ra -sonic -assisted extraction is stable and feasible, and the extraction rate of gallic acid is high from Terminalia chebula .

关 键 词：高效液相色谱 诃子 没食子酸 萃取 

分 类 号：R284[医药卫生—中药学]