中国人群miR-449a单核苷酸多态性与胃癌易感性的相关性研究  

Correlation Study on miR-449a Gene Polymorphism of Single Nucleotide and Susceptibility to Gastric Cancer in A Chinese Han Population

作　　者：张艳梅[1] 马靖华[2] 刘阳晨[3] 刘君[3] 周娟[3] 

机构地区：[1]榆林市第二医院检验科,陕西榆林719000 [2]榆林市第一医院检验科,陕西榆林719000 [3]泰兴市人民医院肿瘤科,江苏泰兴225400

出　　处：《现代检验医学杂志》2016年第4期14-18,共5页Journal of Modern Laboratory Medicine

摘　　要：目的 探讨miR-449a rs112310158基因多态性与胃癌易感性的关系。方法 共纳入448例胃癌患者和452例健康对照者。miR-449a rs112310158基因型检测采用聚合酶链反应结合直接测序方法。miR-449a表达水平检测采用荧光定量PCR方法。此单核苷酸多态性位点功能由RT-PCR和免疫组化试验验证。结果 相对于对照组,胃癌病例组在吸烟者（OR=1.345,95%CI=1.028-1.761）、饮酒者（OR=1.910,95%CI=1.465-2.490）、有肿瘤家族史者（OR=4.178,95%CI=1.554-11.231）、幽门螺杆菌感染率较高者（OR=1.428,95%CI=1.098-1.857）,差异均具有统计学意义。相较AA基因型,GG基因型显著增加胃癌的发病风险（OR=2.542,95%CI：1.304-4.954,P=0.005）。相较于A等位基因,携带G等位基因的个体罹患胃癌的风险升高（OR=1.279,95%CI：1.012-1.617,P=0.043）。GG型患者比AA型患者miR-449a表达水平下降。与AA基因型相比,miR-449a靶基因PRKCE在GG基因型胃癌患者中有更高的表达。结论 该研究发现miR-449a rs112310158是胃癌的遗传易感因素之一。Objective To investigate the relationship of a genetic variant at miR-449a and the risk of gastric cancer. Methods The polymerase chain reaction （PCR） and direct sequencing were used to genotype sequence variants of miR-449a in 448 gastric cancer（GC） cases and 452 controls. The miR-449a levels were measured by quantitative real-time PCR. The function of rs112310158 was confirmed by RT-PCR and immunohistochemistry assay. Results GC cases were more likely to be ciga- rette smokers （OR= 1. 345,95% CI = 1. 028 - 1. 761 ） and alcohol drinkers （OR = 1. 910,95 % CI = 1. 465 - 2. 490）, had cancer history in the first relatives （OR= 4.178,95 % CI = 1.554 - 11.231 ）, and have higher infection rate of H elicobacter pylori （OR= 1. 428,95% CI= 1. 098-1. 857）. Individuals carrying the GG genotypes for the rs112310158 were associated significantly with increased risk of gastric cancer comparing with those carrying wild-type homozygous AA genotypes （OR=2. 542,95% CI： 1. 304-4. 954 ,P= 0. 005）. Furthermore, the G allele increased the risk of occurrence of gastric cancer compared to the A allele （OR= 1. 279,95 Y0 CI： 1. 012 - 1. 617, P = 0. 043）. rs112310158 was associated with the expressionof miR-449a and its target gene. Conclusion A genetic variant at miR-449a may play an important role in the development of gastric cancer.

关 键 词：胃癌 miR-449a 多态性 

分 类 号：R735.2[医药卫生—肿瘤] R730.43[医药卫生—临床医学]