ADAM17基因沉默对肝癌细胞HepG2的VEGFR2自分泌表达的影响  

The regulation of ADAM17 genetic silence to the autocrine expression of VEGFR2 in hepatic carcinoma HepG2 cells

作　　者：刘永存 伍丽萍[2] 李锋[2] 王作仁[2] 

机构地区：[1]咸阳市第一人民医院肿瘤外科,陕西咸阳712000 [2]西安交通大学第一附属医院,陕西西安710061

出　　处：《现代肿瘤医学》2016年第19期3020-3024,共5页Journal of Modern Oncology

摘　　要：目的:观察ADAM17基因沉默对于肝癌细胞HepG2的VEGFR2自分泌表达的调节作用。方法:体外培养肝癌细胞HepG2,采用siRNA的方法靶向沉默HepG2的ADAM17基因,用逆转录PCR和实时定量PCR分析VEGFR2基因水平的变化,用细胞免疫组化法和免疫印迹Western blot法观察VEGFR2蛋白水平的变化。结果:HepG2的VEGFR2蛋白表达在ADAM17基因沉默后72小时受到明显抑制(P<0.05),但是在mRNA水平无明显变化。结论:ADAM17基因对于肝癌细胞HepG2的VEGFR2自分泌表达有重要的调节作用。通过干扰ADAM17基因的表达,可以抑制Hep G2的VEGFR2的蛋白表达水平。ADAM17可以作为肝癌基因治疗的一个靶点。Objective：To observe the regulation of ADAM17 genetic silence on VEGFR2 autocrine expression in HepG2 cells.Methods：HepG2 cells were cultured in vitro and silenced by targeted ADAM17 siRNA,then the varia-tion of VEGFR2 on gene level was analyzed by reverse PCR and Real-time PCR（RT-PCR）,and on protein level by cell immunohistochemistry and Western blot.Results：The protein expression of VEGFR2 in HepG2 cells was mag-nificently suppressed at 72h after ADAM17 gene was silenced（P〈0.05）,while the mRNA expression was little var-ied（P〉0.05）.Conclusion：Gene ADAM17 played an import role in regulating the autocrine expression of VEGFR2 in HepG2 cells.Interfering the expression of ADAM17 could suppress the protein expression of VEGFR2.ADAM17 could be as an genetic target in treatment of hepatic carcinoma.

关 键 词：HEPG2 小干扰RNA 解整合素和金属蛋白水解酶17 血管内皮生长因子受体2 

分 类 号：R735.7[医药卫生—肿瘤]