PAGE5对非小细胞肺癌细胞生长、凋亡和侵袭能力的影响  

The effect of PAGE5 expression on growth,apoptosis and invasion of non- small cell lung cancer cells

作　　者：刘艳琴[1] 刘春红[1] 李娜[1] 史红阳[1] 李维[1] 曹娜娜[1] 黎蒙 

机构地区：[1]西安交通大学第二附属医院呼吸内科,陕西西安710004

出　　处：《现代肿瘤医学》2016年第19期3034-3038,共5页Journal of Modern Oncology

摘　　要：目的:探讨前列腺相关基因5(PAGE5)在非小细胞肺癌(NSCLC)中的表达及其对肺癌细胞生物学行为的影响。方法:采用实时定量PCR和Western blot方法检测37例NSCLC及其癌旁组织中PAGE5的表达;采用MTT法、流式细胞术、Transwell以及Western blot法检测转染PAGE5 siRNA对A549和H1299细胞生长、凋亡、侵袭以及Bax、Bcl-2和MMP-2表达的影响。结果:在37.84%(14/37)的肺癌组织样本中PAGE5基因表达上调。转染PAGE5 siRNA的A549和H1299细胞生长无明显变化、细胞凋亡显著增加、侵袭能力显著下降(P<0.05),Bax蛋白表达显著上调,Bcl-2和MMP-2蛋白表达显著下调(P<0.05)。结论:PAGE5可能通过调控Bax、Bcl-2及MMP-2蛋白的表达影响NSCLC细胞的生长、凋亡与侵袭,有望成为肺癌诊断标志物及潜在的治疗靶点。Objective：To investigate the expression of prostate-associated gene 5 （PAGE5 ）in non-small cell lung cancer（NSCLC）and its biological effect in NSCLC cells.Methods：Quantitative Real -time RT-PCR and Western blot were performed to detect mRNA and protein level of PAGE5 in 37 cases of NSCLC compared with adja-cent non-cancerous lung tissues.MTT assay,flow cytometry,Transwell assay and Western blot were used to evaluate cell growth,apoptosis,invasion and protein level of Bax,Bcl-2 and MMP-2 after transfection of PAGE5 siRNA in A549 and H1299 cells.Results：The expression of PAGE5 in HCC samples was significantly higher than 37.84%（14/37）in adjacent normal lung tissues.Silencing of PAGE5 in A549 and H1299 cells slightly inhibited cell growth, significantly promoted cell apoptosis,depressed cell invasion（P〈0.05 ）,increased Bax and decreased Bcl-2 and MMP-2 protein expression（P〈0.05）.Conclusion：PAGE5 may act as a positive regulator to NSCLC by regulation of Bax,Bcl-2 and MMP-2 protein expression and may be used as a new marker or a potential therapeutic target for NSCLC.

关 键 词：PAGE5 非小细胞肺癌 增殖 凋亡 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]