5-氮-2-脱氧胞苷调控Wif-1基因甲基化对胆管癌细胞侵袭力的影响  

Effect of 5-Aza-2-Deoxycytidine-Regulated Wif-1 Methylation on Invasion of Cholangiocarcinoma Cells

作　　者：刘正人[1] 周水根[1] 聊识君[1] 黄昭明[1] 舒艺[1] 李映良[1] 武彪[1] 

机构地区：[1]南昌大学第一附属医院普外四科,南昌330006

出　　处：《南昌大学学报（医学版）》2016年第4期23-25,55,共4页Journal of Nanchang University：Medical Sciences

基　　金：江西省青年科学基金(20122BAB215022)

摘　　要：目的探讨甲基转移酶抑制剂5-氮-2-脱氧胞苷(DAC)对胆管癌细胞TFK-1侵袭力的影响及其机制。方法采用Transwell法检测胆管癌细胞在不同浓度DAC(0、10、20、40、80、160μmol·L-1)作用下侵袭力的变化;Western-blot法检测DAC作用后Wif-1蛋白的表达。结果 DAC对胆管癌细胞侵袭力有显著性抑制(P<0.05),且呈浓度依赖性;Wif-1蛋白在TFK-1细胞中未见表达,DAC处理后Wif-1蛋白再现表达。结论 DAC对胆管癌TFK-1细胞的侵袭有抑制作用,此作用可能是由Wif-1基因去甲基化来实现。Objective To investigate the effect of DNA methyltransferase inhibitor 5-aza-2-de-oxycytidine （DAC）on the invasion of TFK-1cholangiocarcinoma cells and its mechanism of action.Methods Transwell method was used to detect the invasion of cholangiocarcinoma cells treated with different concentrations of DAC （0,10,20,40,80 and 160 μmol·L^-1 ）.Western-blot method was used to detect the expression of Wif-1 protein.Results DAC inhibited the invasion of cholangiocarcinoma cells in a concentration-dependent manner.The expression of Wif-1 protein was not observed in cholangiocarcinoma cells,but was detected after treatment with DAC.Conclu-sion DAC can inhibit the invasion of cholangiocarcinoma cells,and the mechanism may be in-volved in the demethylation of Wif-1 gene.

关 键 词：5-氮-2-脱氧胞苷 WIF-1 胆管癌 侵袭力 

分 类 号：R735.8[医药卫生—肿瘤]