乳腺癌中THBS-1表达及与EMT的相关性  

Expression of thrombospondins- 1 in the breast carcinoma and the correlation to epithelial- mesenchymal transition

作　　者：廖雪洪 郑雄伟[1,2] 朱伟峰[1] 陈刚[1] 胡丹[1] 张和军[1] 林洁琼[1] 林贤东[1,2] 

机构地区：[1]福建医科大学教学医院,福建省肿瘤医院病理科,福建福州350014 [2]福建省肿瘤转化重点实验室,福建福州350014

出　　处：《现代肿瘤医学》2016年第20期3219-3225,共7页Journal of Modern Oncology

基　　金：国家临床重点专科建设项目;福建省自然科学基金(编号:2014J01302;2016J01508)

摘　　要：目的:检测血小板反应蛋白-1(thrombospondins-1,THBS-1)和EMT相关基因(Snail、E-cadherin及Vimentin)在乳腺癌中的表达情况,探讨THBS-1表达与乳腺癌患者预后及EMT的相关性。方法:应用免疫组织化学方法检测84例乳腺癌石蜡标本及32例对应癌旁组织标本中THBS-1、Snail、E-cadherin及Vimentin表达,分析THBS-1与乳腺癌患者临床病理学资料、预后及EMT的关系。结果:乳腺癌组织中THBS-1蛋白的表达率显著高于癌旁组织,差异有统计学意义(P=0.003);THBS-1蛋白在乳腺癌中的表达与淋巴结转移、NPI及月经状态密切相关(P<0.05);THBS-1高表达组的复发率(23.5%)明显高于THBS-1低表达组(4%),差异具有统计学意义(P=0.009)。THBS-1高表达组的总生存率(65.8%)低于THBS-1低表达组(81.9%)(P=0.062)。THBS-1表达与Snail、E-cadherin及Vimentin密切相关(P<0.05);间质型病例组THBS-1阳性率(58.8%,10/17)明显高于上皮型乳腺癌病例组(21.4%,9/42)(P=0.023),两者差异具有统计学意义。结论:在乳腺癌中THBS-1表达与肿瘤转移密切相关,可能通过诱导EMT过程促进肿瘤的侵袭转移。THBS-1可作为乳腺癌患者判断预后的预测指标。Objective：To evaluate the expression of THBS - 1 and prognosis of breast cancer and its relationship with epithelial - mesenchymal transition （EMT） by expression of THBS - 1,Snail,E - cadherin and Vimentin in the invasive breast cancer. Methods ： The expression of THBS1, Snail, E - cadherin and Vimentin in 84 paraffin - embed-ded tumor tissue and 32 corresponding adjacent tissue was detected using the immunohistochemical method. The rela-tion between expression of THBS - 1 and clinicopathological characteristics, survival and association with EMT were analyzed. Results：The expression rate of THBS -1 in breast cancer was higher than that in the adjacent t issue（P = 0.003）. Moreover,the expression of THBS - 1 in cancer cells was significantly associated with lymph node metasta-sis, Nottingham prognostic index and the menopause （P 〈0. 05） . In Kaplan - Meier analysis,the rate of local regional failure - free survival（ LRFS） for patients in high THBS - 1 expression group（23. 5% ） was significantly higher than that in low THBS - 1 expression group（4% ） ,P =0. 009. Overall survival rate of patients in high THBS -1 expression group （65. 8% ） was lower than that in low THBS -1 expression group （81.9% ） ,P = 0. 062. The expression of THBS - 1 in cancer cells was significantly associated with Snail,E - cadherin and Vimentin （P 〈0. 05）. The expression rate of THBS - 1 in the mesenchymal - like group,with E - cadherin（ - ）/Vimentin（ ＋ ） , （58. 8% , 10/17） was signifi-cantly higher that in the epithelial - like group , with E - cadherin（ - ）/Vimentin（ ＋ ） , （21.4% ,9/42） （P 〈0. 05） . Conclusion ：The expression of THBS - 1 is closely related to metastasis in breast cancer,which might promote tumor metastasis by EMT process. THBS -1 might be used as a predictor for prognosis for breast cancer.

关 键 词：浸润性乳腺癌 THBS-1 免疫组织化学 上皮间质转化 

分 类 号：R737.9[医药卫生—肿瘤]