VCA—IgA与Rta—IgG联合检测诊断鼻咽癌的效能研究  

VCA-IgA and Rta-IgG joint detection diagnosis and effectiveness of nasopharyngeal carcinoma

作　　者：巫翠云[1] 邱梅花[1] 曾晓玲[1] 杜梅英[1] 姚敏[1] 陈一新[1] 

机构地区：[1]海口市人民医院检验科,570208

出　　处：《中华检验医学杂志》2016年第8期609-612,共4页Chinese Journal of Laboratory Medicine

摘　　要：目的探讨VCA—IgA与Rta-IgG联合检测对鼻咽癌诊断的临床价值。方法收集2013年5月至2014年11月在海口市人民医院体检中心3913名健康体检者（组）；男2367名、女1546名。于耳鼻喉科就诊后，经病理活组织检查确诊为鼻咽癌的169例鼻咽癌患者（组）；男118例、女5l例。两组血清标本分别进行EB病毒Rta—IgG、VCA-IgA（ELISA法）检测。组间差异比较用卡方检验，采用ROC曲线评价诊断效能。结果EB病毒Rta—IgG分别为鼻咽癌组93．5％（158／169）、健康体检组2．4％（93／3913）；VCA—IgA阳性率分别为鼻咽癌组79．3％（134／169）、健康体检组8．9％（349／3913）。Rta-IgG诊断鼻咽癌的敏感度93．5％和特异性97．6％，均高于VCA—IgA敏感度79．3％（x。=14．49，P〈0．05）和特异度91．1％（X2=157．15，P〈0．05）。采用VeA-IgA／Rta—IgG联合检测进行分析，未能有效改善鼻咽癌诊断的效果，检测敏感度降低为72．8％（123／169）。结论Rta—IgG的诊断效能优于VCA—IgA。采用VCA—IgA／Rta-IgG联合检测模式对鼻咽癌临床诊断效能未有提高。Objective To investigate the clinical efficacy of combined detection of VCA-IgA and Rta-IgG in the diagnosis of nasopharyngeal carcinoma. Methods From May 2013 to November 2014, 3 913 serum samples（ male 2 367 ,female 1 546 ）from healthy people who had health examination in our medical center were collected and 169 serum samples （male 118, female 51 ） were collected from the patients who were diagnosed as nasopharyngeal carcinoma by pathological biopsy. Serum samples in two groups were detected by EBV RTA-IgG, VCA- IgA assay （ELISA） respectively. SPSS17. 0 statistical software and receiver operating characteristic curve （ROC） were applied to data analysis. Results The Rta-IgG positive rates of EB virus were 93.5% in NPC group （158/169） and 2.4% （93/3 913） in healthy group; while the VCA-IgA positive rates were 79. 3% in NPC group （134/169） and 8.9% （349/3 913 ）in healthy group. The sensitivity （ X2 = 14. 49, P 〈 0. 05 ） and specificity （ X2 = 157.15, P 〈 0. 05 ） of Rta-IgG in the diagnosis of nasopharyngeal carcinoma were significantly better than that of VCA-IgA. Using VCA-IgA/Rta-IgG combined detection analysis, not only failed to effectively improve the diagnosis of nasopharyngeal cancer, but to reduce the detection sensitivity to 72. 8% （123/169）, compared with Rta-IgG detection only. Conclusions Rta-IgG is significantly better than that of VCA-IgA. There was no significant improvement in the clinical diagnostic efficacy of nasopharyngeal carcinoma using VCA-IgA/Rta-lgG combined detection mode.

关 键 词：鼻咽肿瘤 疱疹病毒4型 人 免疫球蛋白A 免疫球蛋白G 衣壳蛋白质类 酶联免疫吸附测定 

分 类 号：R739.63[医药卫生—肿瘤] R730.43[医药卫生—临床医学]