新型光敏剂叶绿酸e6对人膀胱癌5637细胞作用的研究  

Research of photodynamic effect on human 5637 bladder cancer cells mediated by new photosensitizer chlorophyllin e6

作　　者：李锡军 骆磊[2] 李斌[2] 孙立江[2] 张元芳[3] 

机构地区：[1]山东省莱西市人民医院泌尿外科,山东青岛266600 [2]青岛大学附属医院泌尿外科 [3]复旦大学泌尿外科研究所

出　　处：《临床泌尿外科杂志》2016年第8期744-747,共4页Journal of Clinical Urology

基　　金：青岛大学附属医院博士基金资助课题(编号2012)

摘　　要：目的:研究新型光敏剂叶绿酸e6对人膀胱癌5637细胞的杀伤作用,并对其作用机制进行探讨。方法:将5637细胞培养至对数生长期,加入光敏剂叶绿酸e6共同孵育,以650nm激发光照射,MTT法检测叶绿酸e6对5637细胞的杀伤作用,流式细胞术检测细胞凋亡情况。结果:在能量密度为1J/cm2条件下,在2.5、5、10μg/ml浓度时叶绿酸e6对5637细胞的生长抑制率分别为15.92%、43.18%和67.00%,在能量密度为4J/cm2条件下,叶绿酸e6在2.5、5、10μg/ml浓度时对5637细胞的生长抑制率分别为36.78%、72.23%和75.32%;流式细胞仪结果显示5637细胞经光动力作用后,凋亡率为(42.67±1.2)%,与对照组有显著差异。结论:新型光敏剂叶绿酸e6对人膀胱癌5637细胞有明确的杀伤作用,诱导细胞凋亡是可能机制之一。Objective：To investigate new photosensitizer chlorophyllin e6 mediated photodynamic effect on5637 bladder cancer cells,and discuss its potential mechanism.Method：Bladder cancer cells 5637 were incubated to logarithmic phase,and then chlorophyllin e6 was mixed into the culture medium.After the irradiation of 650-nm laser light,the growth inhibition rate was monitored by MTT assay and apoptosis of 5637 cells was detected by flow cytometry.Result：The growth inhibition rates of 2.5,5and 10μg/ml were 15.92%,43.18% and 67.00% respectively,with the optical energy density of 1J/cm2,while with the optical energy density of 4J/cm2,the growth inhibition rates of 2.5,5and 10μg/ml were 36.78%,72.23%and 75.32%respectively.The apoptosis rate of photodynamic treated group was（42.67±1.2）%,which were significantly higher than that of control groups（P〈0.01）.Conclusion：The photodynamic effect of chlorophyllin e6 against 5637cells is definite,and apoptosis may be one of the photodynamic mechanisms.

关 键 词：膀胱肿瘤 光敏剂 叶绿酸 

分 类 号：R737.14[医药卫生—肿瘤]