Rab27b is Involved in Lysosomal Exocytosis and Proteolipid Protein Trafficking in Oligodendrocytes  

作　　者：Yun-Tian Shen Yun Gu Wen-Feng Su Jing-fei Zhong Zi-Han Jin Xiao-Song Gu Gang Chen 

机构地区：[1]Jiangsu Key Laboratory of Neuroregeneration,Co-innovation Center of Neuroregeneration,Nantong University [2]Xinglin College,Nantong University

出　　处：《Neuroscience Bulletin》2016年第4期331-340,共10页神经科学通报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31071251 and 81471255);the National Basic Research Development Program of China(2014CB542202);the Basic Research Program of the Department of Education,Jiangsu Province,China(14KJA310004);a Natural Science Research Project of Nantong Science and Technology Bureau,Jiangsu Province,China(HS2013014);the Natural Science Research Project of Nantong University,Jiangsu Province,China(12Z045,13Z008)

摘　　要：Myelination by oligodendrocytes in the central nervous system requires coordinated exocytosis and endocytosis of the major myelin protein, proteolipid protein （PLP）. Here, we demonstrated that a small GTPase, Rab27b, is involved in PLP trafficking in oligodendrocytes. We showed that PLP co-localized with Rab27b in late endosomes/lysosomes in oligodendrocytes. Short hairpin- mediated knockdown of Rab27b not only reduced lysosomal exocytosis but also greatly diminished the surface expression of PLP in oligodendrocytes. In addition, knockdown of Rab27b reduced the myelin-like membranes induced by co-culture of oligodendrocytes and neurons. Our data suggest that Rab27b is involved in myelin biogenesis by regulating PLP transport from late endosomes/ lysosomes to the cell membrane in oligodendrocytes.Myelination by oligodendrocytes in the central nervous system requires coordinated exocytosis and endocytosis of the major myelin protein, proteolipid protein （PLP）. Here, we demonstrated that a small GTPase, Rab27b, is involved in PLP trafficking in oligodendrocytes. We showed that PLP co-localized with Rab27b in late endosomes/lysosomes in oligodendrocytes. Short hairpin- mediated knockdown of Rab27b not only reduced lysosomal exocytosis but also greatly diminished the surface expression of PLP in oligodendrocytes. In addition, knockdown of Rab27b reduced the myelin-like membranes induced by co-culture of oligodendrocytes and neurons. Our data suggest that Rab27b is involved in myelin biogenesis by regulating PLP transport from late endosomes/ lysosomes to the cell membrane in oligodendrocytes.

关 键 词：MYELINATION Neuron-glial interaction OLIGODENDROCYTE Lysosome Rab27b 

分 类 号：R338[医药卫生—人体生理学]