OPG/RANK/RANKL在牙周炎联合血管钙化大鼠牙髓组织中的表达及意义  

Expression of OPG/RANK/RANKL in the rat dental pulp tissue of periodontitis combined with vascular calcification and its clinical significance

作　　者：潘克清[1] 张朋梅[1,2] 邓婧[1] 娄秀秀 孟芸[3] 刘桂荣[4] 

机构地区：[1]青岛大学附属医院口腔科,山东省口腔临床医学重点实验室,山东青岛266003 [2]青岛慧康医院口腔科,山东青岛266000 [3]天津市东丽区中医医院口腔科,天津300300 [4]青岛市市立医院口腔科,山东青岛266011

出　　处：《上海口腔医学》2016年第4期391-395,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(81100755);山东省口腔生物医学重点实验室开放课题(SDKQ201401)

摘　　要：目的 :研究OPG/RANK/RANKL在牙周炎联合血管钙化大鼠牙髓组织中的表达及其可能的作用。方法 :取36只SPF级雄性Wistar大鼠,随机分为4组,正常对照组(C组)、牙周炎组(CP组)、血管钙化组(VDN组)和牙周炎血管钙化联合组(CP+VDN组),并按照相应的方法建立动物模型。模型建立成功后,取含上颌第二磨牙的上颌骨,H-E染色观察牙髓组织的变化;免疫组织化学Envision法检测牙髓组织中OPG和RANKL蛋白质的表达及OPG/RANKL比值的变化。采用SPSS19.0软件包对数据进行统计学分析。结果:牙髓组织H-E染色观察显示,CP组、VDN组和CP+VDN组牙髓组织不同程度破坏,牙髓内可见中性粒细胞浸润,牙髓血管充血,成牙本质细胞空泡性变,牙髓坏死。其中,CP+VDN组最为显著,其次为CP组、VDN组。免疫组织化学染色结果显示,C组大鼠牙髓组织OPG强阳性表达,CP组、VDN组和CP+VDN组的平均吸光度值显著低于正常对照组(P<0.05),其中以CP+VDN组OPG阳性表达率最低;CP组、VDN组和CP+VDN组大鼠牙髓组织RANKL强阳性表达,各实验组的平均吸光度均显著高于C组(P<0.05),其中CP+VDN组RANKL阳性表达率最高;OPG/RANKL值C组最高,CP+VDN组最低,差异显著(P<0.05)。结论:牙周炎和血管钙化均对牙髓组织产生一定损伤,牙周炎合并血管钙化可加重这一损伤;OPG/RANKL/RANK可能在牙周炎和血管钙化对牙髓组织的影响中发挥重要作用。Abstract：PURPOSE： To study the expression and possible role of OPG/RANK/RANKLin the rat dental pulp of periodontitis combined with vascular calcification. METHODS： Thirty-six male Wister rats were randomly divided into 4 groups： control group（group C）, periodontitis group（group CP）, vascular calcification group（group VDN） and compound group（group CP＋VDN）. Each group underwent corresponding management to establish animal model. When the model was successful, the maxillae including molars were sectioned, pulp tissue was examined by H-E staining; Immunohistochemical staining method was used to evaluate the expression and ratio of OPG and RANKL in pulp tissues. Statistical analysis was carried out using SPSS 19.0 software package. RESULTS： The pulp tissue of group CP, VDN, CP＋VDN showed varied degrees of damage, neutrophil infiltration, pulp vascular congestion, odontoblasts vacuolar changes, pulp necrosis by H-E staining, and the changes in CP＋VDN group was the most significant, followed by CP group, VDN group. Immunohistochemistry showed OPG in pulp tissues in group CP, VDN, CP＋VDN were significantly lower than that in normal group （P〈0.05）, and the expression in group CP＋VDN was the least;Expression of RANKL in pulp tissues in group CP, VDN, CP＋VDN were significantly higher than that in normal group（P〈0.05）,and the expression in group CP＋VDN was the highest. The ratio of OPG/RANKL in normal group was the highest, and the ratio in CP＋VDN group was the lowest. CONCLUSIONS： Periodontitis and vascular calcification can damage the pulp tissue, periodontitis compound with vascular calcification may aggravate the injury; OPG/RANKL/RANK system may play an important role in pulp tissue injury.

关 键 词：牙周炎 血管钙化 牙髓组织 0PG/RANURANKL 

分 类 号：R781.4[医药卫生—口腔医学]