尼妥珠单抗对^(125)I粒子照射人舌鳞癌CAL-27细胞的增敏作用  

The radiosensitization of nimotuzumab on CAL-27 cells after ^(125)I seeds irradiation

作　　者：王兴[1] 辜健敏 孟箭[1] 邵翠玲[1] 

机构地区：[1]徐州市中心医院口腔科,江苏徐州221009

出　　处：《上海口腔医学》2016年第4期409-413,共5页Shanghai Journal of Stomatology

基　　金：江苏省"六大人才高峰"D类资助项目(2012-WS-099);徐州市医学创新(技术攻关)团队(xwcx201604)

摘　　要：目的 :观察尼妥珠单抗联合^(125)I粒子持续低剂量照射对人舌鳞癌CAL-27细胞的抑制效果,初步探讨尼妥珠单抗对^(125)I粒子照射人舌鳞癌CAL-27细胞的放射增敏作用。方法:将指数生长期CAL-27细胞随机分为4组,(空白对照组、^(125)I粒子照射组、尼妥珠单抗组和尼妥珠单抗联合^(125)I粒子照射组),采用CCK-8法检测^(125)I粒子照射组对CAL-27细胞的抑制情况,流式细胞技术测定各组细胞周期分布及细胞凋亡率,Hoechst 33258染色比较观察细胞的形态学变化。采用SPSS17.0软件包对数据进行统计学分析。结果:^(125)I粒子对CAL-27细胞生长有抑制作用且呈时间剂量依赖性。尼妥珠单抗联合^(125)I粒子照射组CAL-27细胞凋亡率显著高于单纯尼妥珠单抗组和单纯照射细胞凋亡率之和,联合组S期细胞比例较对照组显著减少(P<0.05)。结论:尼妥珠单抗联合^(125)I粒子照射可以通过诱导凋亡的途径杀伤CAL-27细胞,且尼妥珠单抗与^(125)I粒子照射释放的低剂量γ射线照射CAL-27细胞具有明显的放射增敏作用。PURPOSE： To observe the inhibitory effect of nimotuzumab combined with low-dose continuous irradiation using 125I seeds on CAL-27 cells originating from human tongue squamous carcinoma, and explore the radiosensitization of nimotuzumab on human tongue squamous carcinoma CAL-27 irradiated with 125I seeds. METHODS： The exponential-phase CAL-27 cells were randomly divided into 4 groups： control group, ^125I seeds irradiation group, nimotuzumab group, nimotuzumab plus ^125I seeds irradiation group. CCK-8 assay was used to detect the inhibitory effect of 125I seeds irradiation on CAL-27 cancer cells, and flow cytometry assays were performed to calculate the apoptosis rate of cells and cell cycle from each group. The morphology of cells was compared using Hoechst 33258 staining. The data were analyzed with SPSS17.0 software package. RESULTS： ^125I seeds inhibited the growth of CAL-27 cells in time-dose dependent manner. The apoptosis rate of cells irradiated by nimotuzumab combined with 125I seeds irradiation was higher than that treated with nimotuzumab and ^125I seeds irradiation respectively, and nimotuzumab followed by radiation had a lower S phase cells（P〈0.05）. CONCLUSIONS： Nimotuzumab combined with ^125I seeds irradiation killed CAL-27 cells by inducing apoptosis. Nimotuzumab and low-dose γ ray emitted from ^125I seeds can significantly enhance the radiosensitivity of CAL-27.

关 键 词：尼妥珠单抗 舌鳞状细胞癌 ^125I粒子 表皮生长因子 放射增敏 

分 类 号：R739.86[医药卫生—肿瘤]