调节性T细胞和白介素15在口腔扁平苔藓中的表达及意义  

Correlation of Treg and IL-15 expression in the peripheral blood of patients with oral lichen planus

作　　者：王翠杰[1] 李言君[1] 薛江南[2] 慈浩粟[1] 李丽萍[1] 李玲[1] 

机构地区：[1]滨州医学院附属医院口腔内科,山东滨州256603 [2]滨州医学院免疫学教研室,山东烟台264003

出　　处：《上海口腔医学》2016年第4期438-442,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(81273200)

摘　　要：目的 :研究外周血CD4^+CD25^+Foxp3^+调节性T细胞(Treg细胞)和白介素15(IL-15)的表达在口腔扁平苔藓(OLP)发病机制中的作用。方法 :采用免疫荧光染色技术,经流式细胞仪检测36例口腔扁平苔藓患者和20例正常人外周血Treg表达水平。应用ELISA技术检测外周血IL-15的表达水平,采用SPSS16.0软件包对其表达差异进行统计学分析。结果:口腔扁平苔藓患者外周血CD4^+CD25^+Foxp3^+Treg细胞数量较正常组显著上升,IL-15表达水平也显著高于正常组,但是口腔扁平苔藓糜烂型与非糜烂型之间的差异无显著性。Treg的表达与IL-15的水平呈正相关(P<0.05)。结论 :Treg和IL-15的表达上升可能是OLP的发病机制之一。PURPOSE： To investigate the expression of Treg and IL-15 in the peripheral blood of patients with oral lichen planus （OLP）. METHODS： The peripheral blood was obtained from 36 OLP patients and 20 healthy controls. Flow cytometry was used to evaluate the proportion of Treg cells, and the level of IL-15 was measured by ELISA. The data were analyzed by SPSS 16.0 software package. RESULTS： In the peripheral blood of OLP patients, the proportion of CD4^＋CD25^＋Foxp3^＋ Treg cells and IL-15 content were significantly higher than those of the healthy controls （P〈0.05）, but there were no significantly difference between two clinical subtypes of OLP（P〉0.05）. The proportion of CD4^＋CD25^＋Foxp3^＋Treg cells was positively correlated with the level of IL-15（r=0.70,P〈0.05）. CONCLUSIONS： The high expression of Treg cells and IL-15 in the perpheral blood of OLP patients may be responsible for the pathogenesis of OLP.

关 键 词：口腔扁平苔藓 调节性T细胞 白介素15 

分 类 号：R781.5[医药卫生—口腔医学]