慢性牙周炎患者治疗前、后血清降钙素基因相关肽水平检测的临床意义  

Clinical significance of calcitonin gene-related peptide level before and after treatment in patients with chronic periodontitis

作　　者：晏莺 向学熔[1,2] 王春[1,2] 叶国[1,2] 范小平[2,3] 杨梅[1,2] 

机构地区：[1]重庆医科大学附属口腔医院牙周病科,重庆401147 [2]口腔疾病与生物医学重庆市重点实验室,重庆401147 [3]重庆医科大学附属口腔医院正畸科,重庆401147

出　　处：《上海口腔医学》2016年第4期448-452,共5页Shanghai Journal of Stomatology

基　　金：重庆市渝中区科技计划项目(20130138);重庆市渝北区科技计划项目[2015(社)10号];重庆市科技委员会计划项目(cstc2014jcyjA10055)

摘　　要：目的 :探讨慢性牙周炎患者治疗前、后血清中降钙素基因相关肽(calcitonin gene-related peptide,CGRP)水平的变化及意义。方法:随机选择2014年8月—2015年6月在我院牙周科就诊的健康对照组30例,轻度、中度、重度牙周炎患者各30例,抽取空腹外周静脉血,离心、分离上清液,运用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测CGRP浓度。慢性牙周炎患者轻度、中度、重度3组分别于基础治疗后3个月再次检测CGRP浓度。采用SPSS 22.0软件包对数据进行统计学分析。结果:ELISA检测结果显示,健康组静脉血中CGRP的含量显著高于牙周炎患者。随着牙周炎炎症程度加重,静脉血中CGRP的含量依次降低,重度牙周炎患者的含量最低,差异显著(P<0.01);轻度、中度、重度牙周炎患者基础治疗3个月后,血清中CGRP含量相比同组治疗前显著升高(P<0.05),治疗后牙周炎患者组间CGRP含量相比差异无显著性(P>0.05)。结论:静脉血中CGRP的含量与慢性牙周炎存在相关性,其含量随慢性牙周炎逐渐加重而降低,推测CGRP可能参与慢性牙周炎的发生、发展过程。慢性牙周炎治疗后血清中CGRP含量明显增加,CGRP可作为慢性牙周炎患者治疗效果的临床检测指标。PURPOSE： To explore the clinical significance of calcitonin gene-related peptide （CGRP） levels in patients with chronic periodontitis before and after treatment, and to detect the calcitonin gene-related peptide content in human venous blood. METHODS： Thirty healthy controls and thirty patients with mild, moderate, severe periodontitis were enrolled from August 2014 to June 2015.CGRP level in the patients＇ peripheral blood was detected by ELISA. Three months after periodontal treatment, CGRP level in mild, moderate, severe periodontitis patients＇ peripheral blood was re-examined by ELISA. Then the correlation between calcitonin gene-related peptide and inflammation of chronic periodontitis was analyzed with SPSS 22.0 software package. RESULTS： The content of CGRP in healthy controls was significantly higher than that in patients with periodontitis. With the aggravation of periodontal inflammation, blood level of CGRP decreased gradually, and the lowest was in patients with severe periodontitis （P〈0.01）. Three months after periodontal treatment, CGRP content was significantly higher compared with that before treatment （P〈0.05）, but no significant difference was found in patients with different degree of periodontitis （P〉0.05）. CONCLUSIONS： The level of CGRP in venous blood decreased with the increasing severity of chronic periodontitis, and CGRP was negatively correlated with the degree of inflammation of chronic periodontitis. CGRP may be involved in the occurrence and development of chronic periodontitis. CGRP content in serum of patients with chronic periodontitis after treatment was significantly increased, CGRP may be used as the basis for clinical detection of chronic periodontitis.

关 键 词：降钙素基因相关肽 慢性牙周炎 酶联免疫吸附法 

分 类 号：R781.42[医药卫生—口腔医学]