女贞子提取物降血糖及抗氧化活性的研究  

Hypoglycemic and anti-oxidant activity of extracts from Ligustrum lucidum

作　　者：赵岩[1] 徐莹[1] 查琳[1] 朱晓富[1] 张连学[1] 郜玉钢[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118

出　　处：《药物评价研究》2016年第3期382-387,共6页Drug Evaluation Research

基　　金：国家公益性行业科研专项(201303111);吉林省科技发展计划项(20140204013YY;20150307012YY)

摘　　要：目的探讨女贞子提取物对糖尿病小鼠的降血糖作用及抗氧化活性。方法以丁羟甲苯(BHT)、维生素C为阳性对照,铁离子还原法、DPPH·自由基清除法测定女贞子提取物体外抗氧化活性;以阿卡波糖为阳性对照,测定女贞子提取物抑制α-葡萄糖苷酶的活性。昆明种小鼠随机分为对照组、模型组、消渴丸(阳性药,1.0 g/kg)组和女贞子提取物低、高剂量(200、800 mg/kg)组,每组8只,以链脲佐菌素(STZ)结合高脂饲料诱导糖尿病小鼠模型,给药治疗3周。每周应用血糖仪检测1次空腹血糖水平;进行口服葡萄糖耐量试验(OGTT);试剂盒法检测血清胰岛素、糖化血红蛋白(Hb A1c)、总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)、谷胱甘肽过氧化物酶(GSH)、超氧化物歧化酶(SOD)、丙二醛(MDA)、尿素氮(BUN)水平。结果女贞子提取物对铁离子还原能力较BHT和Vc差,对DPPH自由基的清除能力较BHT强;对α-葡萄糖苷酶的抑制作用强于阿卡波糖;与模型组比较,女贞子提取物组小鼠血糖水平显著降低,血清TC、TG、LDL-C、Hb A1c、MDA及BUN水平显著降低,胰岛素、HDL-C、SOD和GSH水平显著升高,且存在明显的剂量依赖性。结论女贞子提取物具有显著的降血糖及抗氧化活性。Objective To investigate the protective effects of hypoglycemic and antioxidant activity of extracts from Ligustrum lucidum on experimental diabetic. Methods With dibutyl hydroxy toluene （BHT） and vitamin C as positive control, to determine the in vitro anti-oxidant activity of extracts from L. lucidum by iron reduction and DPPH free radical scavenging method; With acarbose as positive control, to determine the activity of extracts from L. lucidum to inhibit α-glucosidase. Kunming mice were randomly divided into five groups： control group, model group, Xiaoke Pill （positive drug, 1.0 g/kg） group, low- and high-dose （200 and 800 mg/kg） extracts from L. lucidum groups, with eight rats in each group, t;se streptozocin （STZ） combined with high fat diet to establish diabetes model, then mice were treated with drug for 3 weeks. Detect the level of fasting blood glucose by the application of blood glucose meter, do oral glucose tolerance test （OGTT） and detect the levels of insulin, glycosylated hemoglobin （HbAlc）, total cholesterol （TC）, triglyceride （TG）, low density lipoprotein （LDL）, high density lipoprote （HDL-C）, glutathione peroxidase （GSH）, superoxide dismutase （SOD）, malondialdchyde （MDA）, and blood urea nitrogen （BUN） in serum using assay kit. Result The effect of extracts from L. lucidum on the iron reduction was higher than that of BHT and VC, the effect on DPPH. free radical scavenging was stronger than BHT, and the effect on alpha glucosidase inhibition was stronger than that of acarbose. Compared with model group, mice blood glucose levels of extracts from L. lucidum group decreased significantly, serum TC, TG, LDL-C, HbAlc, MDA, and BUN levels decreased significantly, the levels of insulin, high-density lipoprotein cholesterol （HDL-C）, SOD, and GSH were significantly increased, and there was dose dependence. Conclusion Extracts from L. lucidum has significant hypoglycemic and anti-oxidant activity.

关 键 词：女贞子提取物 链脲佐菌素 高脂饲料 降血糖 抗氧化 

分 类 号：R965.1[医药卫生—药理学]