碳源对多粘类芽孢杆菌生长和多粘菌素E合成的影响  

Effect of carbon sources on growth of Paenibacillus polymyxa and polymyxin E synthesis

作　　者：孙仲奇 裘娟萍[1] 陆建卫 赵春田[1] 

机构地区：[1]浙江工业大学生物工程学院,浙江杭州310014 [2]浙江钱江生物化学股份有限公司,浙江海宁314400

出　　处：《浙江农业学报》2016年第8期1343-1350,共8页Acta Agriculturae Zhejiangensis

基　　金：浙江省生物工程重中之重学科开放基金项目(20130103);浙江省自然科学基金项目(Y2111182)

摘　　要：为探究提高多粘菌素E产量的方法,在发酵培养基中分别添加葡萄糖、蔗糖、麦芽糖、乳糖和可溶性淀粉,应用平板菌落计数和HPLC法研究了其对多粘类芽孢杆菌生长和多粘菌素E产量的影响。结果表明,5种碳源对多粘类芽孢杆菌的生长和多粘菌素E产量的影响不同,可溶性淀粉是菌体生长的最适碳源,且多粘菌素E产量依次为可溶性淀粉>乳糖>蔗糖>麦芽糖>葡萄糖。可溶性淀粉有助于延长多粘菌素E的产素期和提高其合成速率,与葡萄糖作碳源相比,多粘菌素E的产量和合成速率分别提高了111%和60%。因此,可溶性淀粉是多粘菌素E合成的最适碳源。In order to explore newmethods to improve the yield of polymy^xin E,five different carbon sources including glucose,sucrose, maltose, lactose and soluble starch were added in the fermentation medium,respectively.The influence on Paenibacillus polymyxa growth and polymyxin E production of different carbon sources were investi-gated through plate counting method and HPLC method. The results indicated that the effects of different carbon sources on the growth of Paenibacillus polymyxa and the polymyxin E production varied significantly. The effect of these carbon sources on polymyxin E production was： soluble starch 〉 lactose 〉 sucrose 〉 maltose 〉 glucose. Soluble starch promoted both cell growth and polymyxin E yield by extending the polymyxin E biosynthetic period and promo-ting the synthetic rate. Compared with glucose as carbon source,the yield and the synthetic rate of polymyxin E were increased by 111% and 60% ,respectively. Therefore,soluble starch was the optimal candidate for carbon sources among the five carbohydrates in polymyxin E synthese.

关 键 词：多粘类芽孢杆菌 多粘菌素E 碳源 可溶性淀粉 

分 类 号：S859.79[农业科学—临床兽医学] Q939.9[农业科学—兽医学]