HPLC测定经不同益生菌发酵前后黄芪中黄芪甲苷和黄芪皂苷Ⅱ的含量  

HPLC based content determination of Astragaloside Ⅵ and Ⅱ in various probiotics fermented Radix Astragali

作　　者：林莎莎[1] 周洪雷[1] 孙筱林 池雨锋 蒋海强[1] 张玲[1] 楚新红 

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]三株医药生物研究所,山东济南250100

出　　处：《山东科学》2016年第4期17-20,49,共5页Shandong Science

基　　金：山东省科技计划(2013GSF11903);山东省自然科学基金(ZR2015HM080);山东省高校中医药抗病毒协同创新课题(XTCX2014C01-03)

摘　　要：采用HPLC-ELSD法测定黄芪经不同益生菌株发酵前后的黄芪甲苷和黄芪皂苷Ⅱ的含量变化。色谱柱为Agilent C_(18)柱(250 mm×4.6 mm,5μm),采用乙腈-水为流动相,梯度洗脱,流速为:1 m L/min。结果表明,黄芪对照中黄芪甲苷和皂苷Ⅱ的含量分别为0.476 2 mg/g和0.050 3 mg/g。用混合菌种发酵后黄芪甲苷和皂苷Ⅱ的含量分别为0.257 8 mg/g和0.109 4 mg/g。黄芪经益生菌发酵后黄芪甲苷含量降低而皂苷Ⅱ的含量升高,且不同菌种发酵后的黄芪甲苷和皂苷Ⅱ的含量也有差异。We determined content variation of AstragalosideVI and Astragaloside II in various probiotics fermented Radix Astragali with HPLC-ELSD. Determination conditions were chromatographic column of Agilent C18 column （250 mm×4.6 mm,5μm）,mobile phase of acetonitrile and water mixture, a linear gradient elution, 1 mL/min flow rate. The contents of AstragalosideVI and Astragaloside II in Radix Astragali were 0.476 2 mg/g and 0. 050 3 mg/g. After fermentation with mixed probiotics, the contents of AstragalosideVI and Astragaloside II were 0. 257 8 mg/g and 0. 109 4 mg/g. Results show that the content of Astragaloside II increases through probiotics fermentation, but the content of AstragalosideVI decreases. Moreover, the contents of Astragaloside VI and Astragaloside II are different through various probiotics fermentation.

关 键 词：黄芪 益生菌发酵 HPLC-ELSD 黄芪甲苷 黄芪皂苷Ⅱ 

分 类 号：R284.1[医药卫生—中药学]