裸鼠原位肝癌移植模型的建立及其生物学观察  

The establishment of orthotopic hepatocellular carcinoma model in nude mice by HepG2 cell line

作　　者：朱瑞东[1] 冯骥良[2] 赵大伟[3] 李宁[1] 

机构地区：[1]首都医科大学附属北京佑安医院普外科中心,100069 [2]首都医科大学附属北京佑安医院临床病理中心,100069 [3]首都医科大学附属北京佑安医院放射科中心,100069

出　　处：《北京医学》2016年第9期921-923,F0003,共4页Beijing Medical Journal

基　　金：国家科技重大专项(2012ZX10002017-007)

摘　　要：目的探讨采用Hep G2细胞株建立裸鼠人肝癌原位模型的方法,评价其生物学特性。方法取人肝癌细胞株Hep G2制备皮下移植瘤,然后将瘤块植入裸鼠肝左叶,建立原位移植模型,观察荷瘤鼠生存状态。裸鼠全身衰竭时处死、取瘤,HE染色及免疫组化法观察瘤组织的病理特点、血管生长及肿瘤细胞凋亡情况。结果种瘤成功率90%,荷瘤鼠中位生存期76.7d,肿瘤直径(16.8±2.2)mm,体积(965.3±333.3)mm^3,重量(1.1±0.4)g。瘤组织早期具有旺盛的增殖能力,晚期癌组织中Caspase3、TUNEL呈强阳性表达。结论由原位移植法建立的裸鼠人肝癌模型,造模时间短、成瘤率高,生物学行为与临床肝癌相似,是基因药物筛选研究的理想模型。Objective To explore the method of establishing orthotopic transplantation tumor model of hepatocellu- lar（HCC） in nude mice by HepG2 cell line. Methods Subcutaneous HCC model was prepared via injecting HepG2 cells into the nude mice. Serially, a piece of tumor block was implanted into the nude mice liver to establish the orthotopic trans- plantation tumor model. The growth status of nude mice, the pathological characteristics of HCC tissues were observed. Results The orthotopic tumor formation rate was 90%. The median survival time of tumor bearing mice was 76.7 d. The tumor diameter, volume and weight was （16.8±2.2） mm, （965.3 ±333.3）mm3, and （1. 1±0.4）g respectively. Tumor tissue had a large number of micro vessels and strong proliferating ability. Capase3, TUNEL was strongly expressed in advanced he- patocellular carcinoma. Conclusion The orthotopic HCC model established in nude mice could imitate the characteristics of human liver cancer cells very well, and it is an ideal animal model for gene therapy study.

关 键 词：肝细胞癌 裸鼠 移植瘤动物模型 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤] R-332[医药卫生—临床医学]