玉米等谷物中玉米赤霉烯酮的高效液相色谱测定法  

Determination of zearalenone in corn and other grain by high performance liquid chromatography

作　　者：张婷[1] 向仲朝[1] 

机构地区：[1]绵阳市疾病预防控制中心,四川绵阳621000

出　　处：《中国卫生检验杂志》2016年第16期2327-2328,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的建立玉米等谷物中玉米赤霉烯酮测定的高效液相色谱法。方法样品用乙腈提取,50℃氮吹干,乙腈定容后进样色谱分析。色谱条件:分离柱为Hypersil C_(18)(250 mm×4.6 mm,5μm);流动相为甲醇-0.02 mol/L乙酸铵(70∶30,V/V);检测波长为236 nm。结果本法在0μg/ml^10μg/ml条件下,线性关系良好(r=0.999 95),ZEN的回归方程为y=81.25x+1.64,方法的检出限为5.0μg/kg。对玉米、小麦、稻谷3类样品分别作了低、中、高3种浓度的加标回收实验,回收率为84.0%~112%,相对标准偏差(RSD)为2.9%~7.1%。结论本法样品处理简单,操作方便,灵敏度高、稳定性好、成本低,可作为谷物中玉米赤霉烯酮定量测定的有效手段。Objective To establish a high performance liquid chromatography（HPLC） method for the determination of zearalenone in corn and other grains.Methods The samples were extracted with acetonitrile,and then blown to driness under flow of nitrogen at 50℃,and finally redisolved in acetonitrile and assayed by chromatography.The chromatographic conditions were： the separation column is Hypersil C（18）（250 mm × 4.6 mm,5 μm）;the mobile phase is methanol-0.02 mol/L ammonium acetate（70： 30,V/V）;the detection wavelength was set at 236 nm.Results Zearalenone showed good linearity when the concentration was within 0 μg/ml-10.00 μg/ml（r=0.999 95）,and the ZEN regressive equation was y=81.25x＋1.64,with the detection limit of 5.0 μg/kg.The recovery rate was within 84%-112% in low,medium and high concentration for samples of corn,wheat and rice.The relative standard deviations was within 2.9%-7.1%.Conclusion This method is simple with easy operation,high sensitivity,good stability and low cost,which can be used as an quantitative determination method for zearalenone in grain.

关 键 词：玉米赤霉烯酮 高效液相色谱法 谷物 

分 类 号：R197.1[医药卫生—卫生事业管理]