缺氧诱导因子-1α的表达在大鼠急性一氧化碳中毒迟发型脑病中的作用  

The expression of hypoxia-inducible factor-1α in hippocampus of rats with delayed encephalopathy after acute carbon monoxide poisoning

作　　者：余倩[1] 邓楠[1] 朱木林[1] 沈流燕 周莉[1] 李经伦 

机构地区：[1]四川省泸州市人民医院神经内科,646000 [2]西南医科大学附属医院神经内科,四川泸州646099

出　　处：《检验医学与临床》2016年第17期2455-2457,2460,共4页Laboratory Medicine and Clinic

摘　　要：目的探讨缺氧诱导因子-1α(HIF-1α)在急性一氧化碳中毒迟发型脑病(DEACMP)中的作用。方法 90只雄性SD大鼠随机分为3组:DEACMP组,空气组(AC组)及空白对照组(BC组)。采用腹腔注射CO法复制DEACMP大鼠模型,选取中毒后第1、3、7、14、21、28天为6个时相点,应用Morris水迷宫实验检测大鼠学习记忆能力,TUNEL法检测大鼠海马区锥体细胞凋亡情况,免疫组织化学染色法检测海马区HIF-1α蛋白的表达情况。结果 DEACMP组大鼠与AC组及BC组比较,平均逃避潜伏期明显延长,第4象限运动时间缩短,差异均有统计学意义(P<0.05)。DEACMP组大鼠海马区神经元凋亡指数于中毒后第3天开始升高,第7天时达到高峰,各时间点凋亡指数明显高于AC组及BC组。HIF-1α在DEACMP组大鼠海马区的表达于中毒后第1天时升高,第3天达到高峰,第28天仍有表达,而且各时间点表达均高于AC组及BC组(P<0.05)。结论 HIF-1α可通过诱导海马区神经元凋亡而参与DEACMP的发病。Objective To observe the effect of hypoxia-inducible factor-1 alpha（HIF-la） in the pathogenesis of delayed encephalopathy after acute carbon monoxide poisoning（DEACMP）. Methods 90 male SD rats（250--320 g） were randomly divided into three groups：blank control group（BC group）, air control group（AC group） and DEACMP group. The time points of 1 st, 3rd, 7th, 14th,21th and 28th day after acute carbon monoxide poisoning were set for measuring the changes. The rats in DEACMP group were injected in abdominal cavity with pure CO several times to establish DEACMP model, rats in AC group were injected with equal air in the same way,BC group was without any treatment. Morris water maze test was used to measure the learning ability and memory. The apoptosis of pyramidal neurons at hippocampal gyrus area was measured by TUNEL test. The expression of HIF-la in hippocampal area was measured by immunohistochemistry. Results The average escaped latency of rats in DEACMP group were increased after poisoning compared with rats in other two groups（P〈0.05）. The time during platform quadrant was shortened in CO group rats after poisoning（P〈0.05）. The apoptosis of pyramidal neurons in hippocampal gyrus was found increased since the 3rd day after the CO poisoning,and it was reached the peak at the 7th day,The apoptotic index in CO group was increased significantly eompare with BC group and AC group （P〈0.05）. The expression of HIF 1α in hippocampal gyrus was found increased since the 1st day after the CO poisoning,reached the peak at the 3rd day, and it still had a high expression at 28th day. The expression in CO group was increased significantly compare with BC group and AC group（P〈0. 05）. Conclusion HIF-1α may play an important role in DEACMP by inducing the apoptosis of neurons in hippocampal gyrus area.

关 键 词：急性一氧化碳中毒迟发型脑病 细胞凋亡 缺氧诱导因子1Α 

分 类 号：R735.34[医药卫生—肿瘤]