青霉素诱导的大鼠癫痫模型中STAT3及IL-6表达  

Expression of STAT3 and IL-6 in epilepsy rat caused by penicillin

作　　者：陈恩[1] 林琳[2,3] 吕赛平[1] 陈杰妮[4] 陈纯静[3] 邹学森[1] 

机构地区：[1]江西省肿瘤医院检验科,南昌330029 [2]厦门医学院基础医学部,厦门361008 [3]中南大学湘雅医学院免疫学系,长沙410078 [4]攀枝花市第二人民医院神经内科,攀枝花617000

出　　处：《中国免疫学杂志》2016年第8期1112-1117,共6页Chinese Journal of Immunology

基　　金：江西省卫生厅中医药科研基金项目(2013B006)资助

摘　　要：目的:通过构建青霉素诱导的大鼠癫痫动物模型,探讨STAT3及IL-6在青霉素诱导的癫痫大鼠模型中的表达。方法:腹腔注射不同剂量的青霉素,制备癫痫动物模型,观察各组大鼠脑电图及行为学,荧光定量PCR方法检测各组动物IL-6及STAT3的mRNA表达量;ELISA法检测血清中IL-6的分泌量;Western blot检测各组动物STAT3的蛋白表达。结果:500 U/kg和800 U/kg青霉素腹腔注射均出现癫痫行为学发作症状及典型的癫痫脑电波,癫痫发作率为100%;和对照组比较,模型组大鼠IL-6以及STAT3 mRNA水平明显升高,血清中IL-6分泌增加,STAT3蛋白表达水平无显著性差异,但p-STAT3蛋白表达水平显著增加。结论:在青霉素诱导的大鼠癫痫模型中,STAT3及IL-6起着重要的作用。Objective： To investigate the expression of IL-6 and STAT3 in the epilepsy rat caused by penicillin. Methods： The model of epilepsy in rats was made by abdominal injecting penicillin. Then the EEG and behavior was observed in each group and the level of IL-6 / STAT3 transcription and cytokine IL-6 secretion in different groups was compared using qRT-PCR and ELISA respectively. The protein expression of STAT3 was detected using Western blot. Results： 500 U / kg and 800 U / kg rats injected penicillin were induced epilepsy and show typical epileptic seizures behavioral and brain waves,seizures was 100%; the level of IL-6 / STAT3 transcription and IL-6 secretion in the model of epilepsy was significantly higher than control. There was no significant difference in the expression of STAT3 protein levels,but a significant increase in p-STAT3. Conclusion： Expression of STAT3 and IL-6 plays an important role in the Epilepsy Rat caused by penicillin.

关 键 词：癫痫 大鼠 STAT3 IL-6 

分 类 号：R741.02[医药卫生—神经病学与精神病学]