TLR7激动剂替代IFN-γ诱导CIK细胞杀伤肿瘤的研究  

Investigation for anti-tumor effects of CIK cells induced by TLR7 agonist instead of IFN-γ

作　　者：李旺 陈艳媛 魏志璋 王宇环 罗晓玲 高东 

机构地区：[1]深圳市合一康生物科技股份有限公司,深圳518045

出　　处：《中国免疫学杂志》2016年第8期1157-1159,共3页Chinese Journal of Immunology

基　　金：深圳市基础研究项目(JCYJ20130326110139687;CXZZ20140509144527788)

摘　　要：目的:探讨TLR7激动剂(Toll like receptor 7 agonist,Tlr7a)替代IFN-γ体外培养细胞因子诱导杀伤性细胞(Cytokine-induced killer cell,CIK)抗肿瘤的免疫效应。方法:分离健康人外周血单个核细胞,在体外诱导CIK。分两组:CIK组和Tlr7a-CIK组。对各组分别进行免疫杀伤性研究,检测细胞免疫表型并分析对K562肿瘤细胞的杀伤活性。结果:CD56+细胞在Tlr7a-CIK组显著增加(P<0.05),Tlr7a-CIK组杀伤活性较CIK组显著增强(P<0.05)。结论:Tlr7a可以替代IFN-γ促进CIK细胞杀伤肿瘤。Objective： To investigate the immune effects of CIK cells induced by Toll like receptor 7 agonist（ Tlr7a） instead of IFN-γ on killing lymphoma cells in vitro. Methods： Mononuclear cells were isolated from healthy human peripheral blood. CIK were induced by Tlr7 a in vitro instead of IFN-γ. Two groups were divided as follows： CIK group,Tlr7a-CIK group. Then the main investigation on immune effects included immune phenotype was detected respectively,and cytotoxicity of the effectors was analyzed. Results： In Tlr7a-CIK group,the amount of CD56^＋cells was more than CIK group（ P〈0. 05）,and the cytotoxicity was also stronger（ P〈0. 05）.Conclusion： Tlr7 a instead of IFN-γ could promote the immune effects of CIK cells on killing tumor cells in vitro.

关 键 词：免疫细胞 Toll样受体7激动剂 抗肿瘤 白血病 

分 类 号：R733.71[医药卫生—肿瘤]