钙蛋白酶抑制剂Calpeptin对乳腺癌MDA-MB-231细胞迁移的影响  

Effect of calpain inhibitor Calpeptin on the migration of MDA-MB-231 breast cancer cells

作　　者：张梦晓[1] 孙小锦 张智瑞[1] 潘琼[1] 赵素容[1] 刘浩[1] 

机构地区：[1]蚌埠医学院药学系安徽省生化药物工程技术研究中心,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2016年第7期841-844,共4页Journal of Bengbu Medical College

基　　金：国家自然科学基金项目(81372899);蚌埠医学院自然科学基金项目(Byky1447)

摘　　要：目的:探讨钙蛋白酶抑制剂Calpeptin对乳腺癌MDA-MB-231细胞迁移能力的影响,为治疗乳腺癌转移提供思路。方法:MTT法检测不同浓度Calpeptin对MDA-MB-231细胞增殖的影响;细胞划痕实验及Transwell迁移实验检测不同浓度的Calpeptin对MDA-MB-231细胞迁移的影响;Western blot法检测不同浓度Calpeptin对MDA-MB-231细胞基质金属蛋白酶-2表达的影响。结果:Calpeptin可抑制MDA-MB-231细胞的增殖,其增殖抑制作用随浓度的增大而增加(P<0.01)。Calpeptin作用MDA-MB-231细胞24 h后,细胞的迁移能力明显下降(P<0.01)。Western blot结果显示,Calpeptin可下调MDA-MB-231细胞中细胞基质金属蛋白酶-2的表达。结论:Calpeptin能够抑制乳腺癌MDA-MB-231细胞迁移,其机制可能与下调MMP-2的表达有关。Objective： To investigate the effects of calpain inhibitor Calpeptin on the migration of MDA-MB-231 breast cancer cells for providing an idea in treating the metastatic breast cancer. Methods： The effects of different concentrations of Calpeptin on the proliferation of MDA-MB-231 cells were determined using MTT assay. The effects of different concentrations of Calpeptin on the migration of MDA-MB-231 cells were detected using the wound healing assay and transwell migration assay. The effects of different concentrations of Calpeptin on the expression of matrix metalloproteinase-2（ MMP-2） in MDA-MB-231 cells were detected using Western blot. Results： Calpeptin could suppress the proliferation of MDA-MB-231 breast cancer cells,the inhibiting effects of which increased with the increasing of the concentrations of Calpeptin（ P〈0. 01）. The migration ability of MDA-MB-231 cells decreased significantly after 24 h of treatment with Calpeptin（ P〈0. 01）. The result of Western blot showed that Calpeptin could down-regulate the expression of MMP-2 in MDA-MB-231 cells. Conclusions： Calpeptin can inhibit the migration of MDA-MB-231 cells,the mechanism of which may involve in the down-regulation of MMP-2.

关 键 词：乳腺肿瘤 MDA-MB-231细胞 钙蛋白酶 Calpeptin 迁移 细胞基质金属蛋白酶-2 

分 类 号：R737.9[医药卫生—肿瘤]