肉桂醛对肝癌HepG2细胞p21和CDK4蛋白的影响  

Effect of cinnamaldehyde on the expression of p21 and CDK4 in human hepatoma cell line HepG2

作　　者：王旭林[1] 王萍[1] 侯玉龙[1] 李明[1] 刘蕾[2] 

机构地区：[1]佳木斯市中心医院肿瘤科,黑龙江佳木斯154007 [2]佳木斯大学基础医学院病理生理学教研室,黑龙江佳木斯154007

出　　处：《实用肿瘤杂志》2016年第4期344-348,共5页Journal of Practical Oncology

摘　　要：目的检测肉桂醛对肝癌HepG2细胞p21和细胞周期依赖性蛋白激酶4(cyclin-dependent kinase 4,CDK4)蛋白表达的影响。方法肉桂醛(3.13、1.56、0.78、0.39、0.20、0.10 g/L)作用肝癌HepG2细胞后,MTT比色法检测HepG2细胞增殖活性和半数抑制浓度(half maximal inhibitory concentration,IC_(50))。流式细胞术检测肉桂醛对HepG2细胞凋亡影响。免疫荧光法检测HepG2细胞p21和CDK4蛋白的表达。结果肉桂醛能抑制HepG2细胞增殖,且呈剂量和时间依赖性,24小时、48小时和72小时IC_(50)值分别为0.68、0.45、0.35 g/L(P<0.01)。肉桂醛组(0.900、0.450、0.225 g/L)肝癌HepG2细胞凋亡率高于对照组,差异有统计学意义(P<0.01)。肉桂醛能增加p21蛋白和降低CDK4蛋白在HepG2细胞中的表达,各浓度肉桂醛组的p21、CDK4蛋白表达与对照组比较,差异均有统计学意义(均P<0.05)。结论肉桂醛可抑制人肝癌细胞HepG2的增殖,可能与调节p21和CDK4的蛋白表达有关。Objective To investigate the effect of cinnamaldehyde on the expression of p21 and CDK4 protein in the human hepatoma cell line HepG2. Methods MTT assay was used to test the proliferation activity and half growth inhibition concentration（ IC50） of HepG2 cells after cinnamaldehyde treatment（ 3. 13,1. 56,0. 78,0. 39,0. 20,0. 10 g/L）; Fluorescence Activating Cell Sorter（ FACS） was used to detect the apoptosis status of HepG2; Immunofluorescence staining was used to detect the expression of p21 and CDK4 protein in HepG2 cells. Results Cinnamaldehyde inhibited HepG2 proliferation in a dosage- and time-dependent manner. The IC50 were 0. 68 g / L of 24 h,0. 45 g / L of 48 h and 0. 35 g / L of 72 h respectively（ P〈0. 01）. The apoptotic rates of HepG2 were higher in the cinnamaldehyde（ 0. 900,0. 450,0. 225 g / L）treatment groups,compared with the control group（ P〈0. 01）. Cinnamaldehyde increased the expression of p21 protein and decreased the expression of CDK4 protein in HepG2 cells. In all treatment groups,the expression of p21 and CDK4 protein were significantly different,compared with the control group（ P 〈0. 05）. Conclusion Cinnamaldehyde inhibits the proliferation of HepG2 cells,which may be related to its regulations on the expression of p21 and CDK4.

关 键 词：肝肿瘤/药物疗法 细胞周期蛋白依赖激酶4 原癌基因蛋白质p21(ras) 丙烯醛/药理学 肿瘤细胞 培养的/药物作用 

分 类 号：R735.7[医药卫生—肿瘤] R730.53[医药卫生—临床医学]