CCM联合HCPT对人膀胱癌BIU-87细胞YAP、TEAD1表达及生物学功能的影响  

Effects of curcumin combined with hydroxycamptothecin on the expression of YAP,TEAD1 gene and biological function in human bladder cancer cell line BIU-87

作　　者：何江[1] 曹建佳 陈柯宏 王德林[2] 盛夏[2] 李文宾[2] 黄亮亮[2] 

机构地区：[1]重庆医科大学附属大学城医院消化神经中心,401331 [2]重庆医科大学附属第一医院泌尿外科,400016

出　　处：《重庆医学》2016年第25期3462-3465,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(30972999)

摘　　要：目的探讨姜黄素(CCM)联合羟基喜树碱(HCPT)对人膀胱癌BIU-87细胞中YAP、TEAD1表达及凋亡的作用。方法 CCM和HCPT分别单独与联合用药处理膀胱癌BIU-87细胞。免疫组织化学检测膀胱癌组织及癌旁组织中YAP和TEAD1表达情况,流式细胞仪检测细胞凋亡情况;RT-PCR和Western blot检测YAP及TEAD1基因的mRNA和蛋白的表达变化。结果 YAP和TEAD在膀胱癌组织及癌旁组织中均有阳性表达,差异有统计学意义(P<0.05)。流式细胞仪检测结果显示CCM和HCPT均可有效诱导膀胱癌细胞BIU-87的凋亡。CCM组和HCPT组BIU-87细胞中YAP及TEAD1mRNA和蛋白表达显著降低(P<0.05),联合用药组YAP及TEAD1mRNA和蛋白表达更低(P<0.05)。结论 CCM和HCPT联合用药抗癌机制可能是通过抑制Hippo信号通路中YAP癌基因表达,下调TEAD1,并诱导膀胱癌细胞凋亡产生。Objective To investigate the expression of curcumin （CCM） combined with hydroxycamptothecin （HCPT） on YAP,TEAD1 and apoptosis in human bladder cancer cell line BIU-87. Methods CCM and HCPT were separately treatment and combined treatment of bladder cancer BIU-87 cells. Immunohistochemical（IHC） method was used to detect expression of YAP and TEAD1 in bladder cancer and tissue adjacent to carcinoma：Apoptosis rate of the human bladder cancer cell BIU-87 was detected by flow cytometry（FCM） instruments after CCM and HCPT were administered alone or in combination; Detection of YAP, TEAD1 gene mRNA and protein expression changes was depended on RT-PCR and Western blot. Results The results showed that YAP and TEAD1 had positive expressions in bladder cancer and tissue adjacent to carcinoma （P〈00.05） .the difference had obvious sig- nificance. The results of detection by FCM showed that CCM and HCPT both induced apoptosis of bladder cancer cell BIU-87 effec- tively. The mRNA and protein expression of YAP and TEAD1 in human bladder cancer cell BIU-8 significantly reduced in both CCM group and HCPT group,and mRNA and protein expression oF YAP and TEAD1 was lower in the combination group（P〈 0.05）. Conclusion CCM and HCPT combination anti-cancer mechanism may be through inhibiting the Hippo signaling pathway in YAP cancer gene expression,cut TEAD1 ,and induce bladder cancer cell apoptoms.

关 键 词：姜黄素 膀胱肿瘤 羟基喜树碱 YAP TEAD1 

分 类 号：R737.14[医药卫生—肿瘤]