维生素D受体SNPs基因多态性与新疆汉族2型糖尿病视网膜病变相关性研究  

Association between vitamin D receptor gene polymorphisms in 4 SNPs and diabetic retinopathy of type 2 diabetes mellitus in Han population in Xinjiang

作　　者：李丽[1] 易湘龙[2] 钱一[2] 周维[2] 赵春焕[2] 梅波[2] 

机构地区：[1]新疆医科大学第五附属医院眼科,乌鲁木齐830011 [2]新疆医科大学第一附属医院眼科,乌鲁木齐830054

出　　处：《新疆医科大学学报》2016年第9期1081-1085,共5页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(81260150,8156016I);国家教育部高等学校博士学科点专项科研基金新教师类(20126517120004);中国博士后科学基金面上资助项目(2014M562485);新疆医科大学第一附属医院青年科研基金(2012QN08)

摘　　要：目的探讨维生素D受体（VDR）BsmⅠ、ApaⅠ、FokⅠ、TaqⅠ基因多态性与新疆汉族2型糖尿病视网膜病变的关系。方法 589例新疆汉族T2DM患者入选,其中非增殖性糖尿病视网膜病变患者181例,增殖性糖尿病视网膜病变患者146例,无糖尿病视网膜病变患者262例。抽取血液样本提取DNA。采用iMLDR技术进行VDR基因BsmⅠ、ApaⅠ、FokⅠ、TaqⅠ位点基因分型,计算等位基因及基因型并进行χ^2检验。结果 3组rs1544410、rs7975232、rs2228570及rs731236基因型及等位基因分布频率比较差异均无统计学意义（P〉0.05）。结论 VDR基因BsmⅠ、ApaⅠ、FokⅠ及TaqⅠ4个SNPs的多态性与新疆汉族2型糖尿病视网膜病变无关。Objective To investigate association between Bsm I , Apa I , Fok I and Tag I polymorphisms in vitamin D receptor （VDR） gene and diabetic retinopathy （DR） of type 2 diabetes melli- tus （T2DM） in Han population in Xinjiang. Methods 589 patients of T2DM in Han population were screened, including 181 patients with nonproliferative retinopathy （NPDR）, 146 patients with proliferative retinopathy （PDR）, and 262 patients without diabetic retinopathy. DNA was extracted from blood samples. Bsrn I , Apa I , Fok I and Taq I polymorphisms in VDR gene were genotyped using iMLDR technique. Allele and genotype frequencies were compared among three groups using the X2 test. Results The genotype and allele frequencies of rs1544410, rs7975232, rs2228570 and rs731236 were not different among three groups （P 〈0.05）. Conclusion There were no correlation between Bsm I , Apa I , Fok I , and Taq I polymorphisms in VDR gene and DR of T2DM in Han population in Xinjiang.

关 键 词：糖尿病视网膜病变 维生素D受体 基因多态性 

分 类 号：R77[医药卫生—眼科] R34[医药卫生—临床医学]