双波长薄层色谱扫描法测定刺糖中蔗果三糖的含量  

Determination of GF2 in Alhagi-honey by dual wavelength TLC-scanning

作　　者：谢睆 施洋[2] 常军民[1] 

机构地区：[1]新疆医科大学药学院,乌鲁木齐830011 [2]新疆医科大学学报编辑部,乌鲁木齐830011

出　　处：《新疆医科大学学报》2016年第9期1163-1165,1169,共4页Journal of Xinjiang Medical University

基　　金：新疆医科大学创新基金(XJDCX201407);新疆维吾尔自治区科技援疆计划(201491172)

摘　　要：目的建立双波长薄层色谱扫描法测定不同时间、产地刺糖中蔗果三糖（GF2）含量。方法以GF2为标准品,采用双波长薄层扫描分析,展开剂为冰乙酸-三氯甲烷-95%乙醇（9∶11∶11）,显色剂为二苯胺-苯胺-丙酮-磷酸（1∶1∶50∶5）,最大吸收波长510nm,参比波长610nm。结果蔗果三糖（GF2）在0.642 8～15.975 2μg范围内线性关系良好,r=0.999 2,平均回收率为102.5%,RSD为1.55%。不同产地刺糖中GF2含量为在12.957 3～36.328 1mg/g。结论双波长薄层色谱扫描法分离效果好,操作简便,可用于刺糖中GF2的定性及定量分析。Objective To establish the method for the determination of kestose in different time and origin of Alhagi-honey by Dual Wave length TLC-Scanning.Methods Dual Wavelength TLCS canner was selected with GF2 as standard substance.The developing solvent was glacial acetic acid-trichloromethane-95% ethanol（9∶11∶11）.The color developing agent was diphenylamine-aniline-acetone-phosphoric acid（1∶1∶50∶5）;λS=510nm,λR=610nm.Results The calibration curve was liner in range of 0.642 8-15.975 2μg for kestose（r =0.999 2）.The average recovery was 102.5%,and RSD was 1.55%.The content of GF2 from different places of origin habitats in Alhagi-honeywere between 12.957 3-36.328 1mg/g.Conclusion The established method has a good separation effect and simple operation,so can be used for the qualitative and quantitative analysis of kestose from Alhagi-honey.

关 键 词：刺糖 蔗果三糖 双波长薄层扫描法 

分 类 号：R914[医药卫生—药物化学]