Stereotactic Injection of shRNA GSK-3β-AAV Promotes Axonal Regeneration after Spinal Cord Injury  

作　　者：左玉超 熊南翔 赵洪洋 

机构地区：[1]Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology [2]Department of Neurosurgery, the First Affiliated Hospital of Zhengzhou University

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2016年第4期548-553,共6页华中科技大学学报（医学英德文版）

基　　金：supported by the National Natural Science Foundation of China(No.81371380)

摘　　要：Evidence suggested that glycogen synthase kinase-3β（GSK-3β） is involved in Nogo-66 inhibiting axonal regeneration in vitro, but its effect in vivo was poorly understood. We showed that stereotactic injection of sh RNA GSK-3β-adeno associated virus（GSK-3β-AAV） diminished syringomyelia and promoted axonal regeneration after spinal cord injury（SCI）, using stereotactic injection of sh RNA GSK-3β-AAV（tested with Western blotting and RT-PCR） into the sensorimotor cortex of rats with SCI and by the detection of biotin dextran amine（BDA）-labeled axonal regeneration. We also determined the right position to inject into the sensorimotor cortex. Our findings consolidate the hypothesis that downregulation of GSK-3β promotes axonal regeneration after SCI.Evidence suggested that glycogen synthase kinase-3β（GSK-3β） is involved in Nogo-66 inhibiting axonal regeneration in vitro, but its effect in vivo was poorly understood. We showed that stereotactic injection of sh RNA GSK-3β-adeno associated virus（GSK-3β-AAV） diminished syringomyelia and promoted axonal regeneration after spinal cord injury（SCI）, using stereotactic injection of sh RNA GSK-3β-AAV（tested with Western blotting and RT-PCR） into the sensorimotor cortex of rats with SCI and by the detection of biotin dextran amine（BDA）-labeled axonal regeneration. We also determined the right position to inject into the sensorimotor cortex. Our findings consolidate the hypothesis that downregulation of GSK-3β promotes axonal regeneration after SCI.

关 键 词：NEURITES wound healing protein-serine-threonine kinases myelin proteins 

分 类 号：R651.2[医药卫生—外科学]