Snail在海人酸诱导癫痫持续状态小鼠皮层中高表达  

High expression of snail in mouse cerebral cortex following kainic acid-induced status epilepticus

作　　者：王静[1,2] 王法祥[3] 杨云鹏[2] 吴海琴[1] 

机构地区：[1]西安交通大学第二附属医院神经内科,陕西西安710004 [2]西安市第四医院神经内科,陕西西安710004 [3]第三军医大学新桥医院神经内科,重庆460000

出　　处：《细胞与分子免疫学杂志》2016年第9期1193-1196,1201,共5页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的观察snail因子在海人酸(KA)诱导癫痫持续状态(SE)小鼠皮层中的表达和分布,探讨snail在癫痫持续发作状态中的作用。方法成年雄性C57/BL6小鼠64只,随机分成对照组和SE组,每组32只。对照组采用腹腔注射生理盐水,SE组采用KA腹腔注射诱发小鼠癫痫急性发作,并且各组分别在发作终止后的3、8、24、72 h进行取材。反转录PCR检测snail的mRNA表达水平,Western blot法检测snail的蛋白表达水平,免疫组织化学检测snail的形态分布,免疫荧光技术检测snail的定位。结果与对照组相比,SE组snail mRNA和蛋白的表达水平明显上升,24 h达到高峰;免疫组织化学技术显示,在对照组,snail表达呈弱阳性。在SE组,snail在不同皮层神经元以及胶质状细胞都有广泛分布;免疫荧光技术进一步证明snail在神经元和星形胶质细胞均有表达。结论 Snail在KA诱导SE小鼠皮层神经元和星形胶质细胞内都有强表达,提示snail可能与癫痫的发作有一定的联系。Objective To observe the expression of snail in mouse cortex in the model of status epilepticus （SE） induced by kainic acid （KA） and explore the role of snail in SE. Methods Sixty-four adult male C57/BL6 mice were randomly divided into control group and SE group, 32 mice in each group. Intraperitoneal injection of normal saline was performed in control group; instead, KA was injected intraperitoneally in SE group to induce SE. The organs were harvested 3, 8, 24 and 72 hours post the cessation of each group. Reverse transcription-PCR was used to detect the expression of snail mRNA; the expression of snail protein was tested by Western blotting; the distribution of snail was determined using immunohistochemistry; snail expression in cortex was located by immunofluorescence technique. Results Compared with the control group, mRNA and protein expression of snail in SE group were significant higher, and they reached the peak at 24 hours. Immunohistochemistry indicated weak positive expression of snail in control group. In SE group, it was widely distributed in different cortical neurons and glial cells. Immunofluorescence histochemistry further proved that snail was localized in neurons and astrocytes. Conclusion Snail is strongly expressed in neuron and astrocyte of mouse cortex at KA-induced SE, which indicates snail is possibly related to the attack of SE.

关 键 词：海人酸 癫痫持续状态 SNAIL 皮层 表达 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R749[医药卫生—临床医学]