半乳糖凝集素-1对急性脊髓损伤模型大鼠的保护作用及其机制  

Protective effects and its mechanism of Galectin-1 on the rats of acute spinal cord injury

作　　者：陈颖东[1] 宋清宇[1] 谢景泉 

机构地区：[1]广州市第八人民医院神经外科,510060 [2]湛江市第二人民医院外科

出　　处：《临床神经外科杂志》2016年第3期176-180,共5页Journal of Clinical Neurosurgery

基　　金：广东省医学科研基金立项(A2012254)

摘　　要：目的研究半乳糖凝集素-1(Galectin-1)对大鼠急性脊髓损伤的保护作用及其机制。方法 45只SPF级SD大鼠随机平均分为Galectin-1组、甲泼尼龙(MP)组和生理盐水对照组(生理盐水组)。以Allen's法打击脊髓造模,分别在术后1 d、7 d、14 d每组各取5只大鼠,先做BBB评分及斜板试验,然后处死大鼠取损伤处脊髓组织进行苏木精-伊红(HE)染色及胶质纤维酸性蛋白(GFAP)免疫组化染色检测,同时用酶联免疫吸附测定(ELISA)法检测血清白细胞介素-10(IL-10)的含量。结果术后1 d,3组大鼠BBB评分、斜板试验结果及血清IL-10含量的差异无统计学意义(均P>0.05);术后7 d、14 d,MP组和Galectin-1组的BBB评分、斜板试验结果及血清IL-10含量均较生理盐水组高(均P<0.05),而MP组与Galectin-1组之间的差异均无统计学意义(均P>0.05)。HE染色显示术后各时间点,Galectin-1组和MP组脊髓损伤肿胀及出现核固缩或解离神经元的数量少于生理盐水组。术后1 d,3组大鼠脊髓星形胶质细胞(GFAP表达阳性)数量无明显区别;术后7 d、14 d,Galectin-1组及MP组明显多于生理盐水组,而Galectin-1组与MP组则无明显差别。结论Galectin-1对大鼠急性脊髓损伤具有一定的保护作用,其机制可能是通过增强机体IL-10的表达,从而抑制损伤后的炎症反应而发挥脊髓保护作用的。Objective To study the rats of acute spinal cord injury. Methods protective effects and its mechanism of Galectin-1 on the 45 SPF SD rats were randomized to Galectn-1 group, methylprednisolone（MP） group, normal saline injection group with 15 rats in each group. After modeling success,5 rats of each group after 1 d,7 d, 14 d, are made the score of BBB and inclined plate experiment, and then the rats were sacrificed and the wound spinal cord tissue hematoxyhn- eosin staining （HE） staining and immunohistochemical GFAP, and serum interleukin-10 （IL-10） concentration of each rat were detected by enzyme-linked immunosorbent assay （ELISA）. Results On postoperative day first rats in each group BBB score and the inclined plate score and the inclined plate test showed no significant difference （P 〉 0.05 ） ;after 7d and 14d ,the BBB in group MP and group Galectin-1 BBB score test results in the MP group and Galectin-1 group are higher than those in the injury group （ P 〈 0.05 ） ; MP group and Galectin-1 group were no significant difference between the data （ P 〉 0.05 ）. HE staining showed ： 1 d after the operation of each rat neuronal swelling, few neurons staining becomes sparse;after 7d in rat spinal cord cell edema reached a peak, the swelling is very obvious, some neurons showed nuclear pyknosis, cell body shrinkage, the extracellular space is increased, some nuclear dissociation; experiment 14d rat neurons decreased in number, but the surviving neurons envelope gradually clear, swelling. HE staining showed injury at different time points, Galectin-1 group and MP group injury swelling and karyopyknosis or dissociation of the neuronal number is less than those in the injury group. GFAP immunohisto- chemistry showed ： 1 d after the operation, each rat astrocyte GFAP staining showed no difference ; after 7 d, 14d,group Galectin-1 and group MP was higher than the injury group,GaIectin-1 group and MP group showed no significant difference. The concentration of IL-10 in serum of rat betw

关 键 词：GALECTIN-1 大鼠 脊髓损伤 神经保护作用 

分 类 号：R826.65[医药卫生—临床医学]