FRK通过FAK信号通路抑制脑胶质瘤细胞迁移与侵袭作用的研究  

FRK inhibits human glioma cells invasion and migration through FAK regulation

作　　者：王军[1] 石琼[2,3] 张春亭[1] 金戈[1] 张道为 宋旭[3] 周秀萍[2] 于如同[2,3] 

机构地区：[1]徐州医学院研究生院,徐州221000 [2]徐州医学院神经系统疾病研究所 [3]徐州医学院附属医院脑科医院神经外科

出　　处：《临床神经外科杂志》2016年第4期263-266,271,共5页Journal of Clinical Neurosurgery

基　　金：国家自然科学基金(基金编号:81272777;81372699;81472345;81402046)

摘　　要：目的研究酪氨酸相关激酶(fyn-related kinase,FRK)是否通过粘着斑激酶(focal adhesion kinase,FAK)信号通路抑制脑胶质瘤细胞的迁移和侵袭。方法采用Western blot(WB)检测FRK质粒的转染效果,细胞划痕和Transwell侵袭实验检测过表达FRK及下调FAK对胶质瘤U251细胞迁移和侵袭能力的影响;WB检测过表达FRK对FAK、P-FAK蛋白水平的影响以及下调FAK对FRK蛋白水平的影响。结果FRK质粒转染成功。过表达FRK后胶质瘤U251细胞的迁移与侵袭能力下降。FAK siRNA干扰效率达到70%,下调FAK后胶质瘤U251细胞的迁移与侵袭能力下降。过表达FRK后P-FAK的蛋白水平明显下降,而FAK蛋白水平无明显变化,下调FAK对FRK的蛋白水平无明显影响。结论 FRK可能通过抑制FAK的磷酸化来调控胶质瘤的侵袭与迁移。Objective To explore the mechanism of the inhibitory effect of fyn-related kinase （ FRK） on the glioma cells migration and invasion through focal adhesion kinase （ FAK） signaling pathways .Methods Western blot was used to examine the transfection efficiency of FRK plasmid . The effect of FRK over-expression or FAK down-regulation on the glioma U251 cells migration and invasion ability was examined by wound healing assay and transwell invasion assay respectively .The effect of FRK over-expression on the protein level of FAK , P-FAK, and the influence of FAK down-regulation on FRK protein level were analyzed by WB .Results The FRK plasmid was transfected into glioma U251 cells successfully , and FRK over-expression decreased the glioma cells migration and invasion .The efficiency of FAK reached to about 70%, and FAK down-regulation decreased the migratory and invasive ability of glioma U 251 cells.FRK over-expression decreased the protein level of P-FAK significantly , but had no effect on the total protein levels of FAK .In addition, the protein levels of FRK had no change after FAK down-regulation .Conclusion FRK may regulate the migration and invasion of the glioma cells by dephosphorylating the focal adhesion kinase .

关 键 词：胶质瘤 迁移与侵袭 酪氨酸相关激酶 粘着斑激酶 

分 类 号：R739.41[医药卫生—肿瘤]