MiR-125a诱导表达与顺铂抵抗的鼻咽癌细胞株凋亡相关性的研究  

The relationship between MiR-125a induction expression and apoptosis of cisplatin resistant nasopharyngeal carcinoma cell line

作　　者：胡琼英[1] 艾承锦[1] 张爽[1] 熊大千 江泽友[1] 赵梓亦[2] 张朝明[1] 

机构地区：[1]成都中医药大学附属医院检验科,四川成都610071 [2]成都中医药大学附属医院科研部中心实验室,四川成都610071

出　　处：《实用医院临床杂志》2016年第5期43-46,共4页Practical Journal of Clinical Medicine

基　　金：成都中医药大学科技发展基金资助项目(编号:030029045)

摘　　要：目的探讨顺铂抵抗的鼻咽癌细胞株TW03微小RNA-miR-125a表达情况,为顺铂治疗鼻咽癌的耐药性寻找新的分子靶点和标志物。方法建立顺铂抵抗的鼻咽癌细胞株TW03细胞模型(TW03/DDP),提取细胞中的总RNA,实时荧光定量聚合酶链反应(reverse transcription-quantitative polymerase chain reaction,RT-q PCR)分析该细胞株中miR-125a表达情况,过表达miR-125a和anti-miR-125a后,检测TW03细胞的凋亡和增殖情况。结果顺铂抵抗的鼻咽癌细胞株中miR-125a的表达增高,后者可抑制顺铂抵抗的鼻咽癌细胞株凋亡。结论顺铂抵抗的鼻咽癌细胞株诱导表达miR-125a可抑制鼻咽癌细胞株凋亡。Objective To search the novel molecule targets or biomarkers for cisplatin resistant patients with nasopharyngeal carcinoma （NPC） ,we detected the expression levels of miR125a in cisplatin resistant TW03 cells （TW03/DDP）. Methods After construction of a cisplatin resistant TW03 cell model （TWO3/DDP）, the expression levels of miR125a were examined by RT-qPCR. After over expression of miR-125a and application of anti-miR-125a, the apoptosis and proliferation of TW03 cells were examined. Results In the TW03/DDP cells ,the expression levels of miR125a were upegulated. The cisplatinindueed expression of miR125a inhibited apoptosis of the TWO3 cells. Conclusion The present study revealed that induction of the expression of miR125a by treatment with cisplatin results in resistance to the eisplatin drug in the NPC cells.

关 键 词：鼻咽癌 顺铂抵抗 miR-125a 凋亡 

分 类 号：R739.62[医药卫生—肿瘤]