高压脉冲电场对小球藻破碎效果的影响  

Influence of High Voltage Pulsed Electric Fields on Disrupture of Chlorella

作　　者：张若兵[1] 傅贤[1] 寇梅如 

机构地区：[1]清华大学深圳研究生院,深圳518055

出　　处：《高电压技术》2016年第8期2668-2674,共7页High Voltage Engineering

基　　金：国家高科技研究发展计划(863计划)(2011AA100801);国家自然科学基金(51177082);深圳市科技计划(JCYJ20120616220833092)~~

摘　　要：含油微藻的破碎是微藻制油过程中的重要环节。为此,利用清华大学自主研制的高压脉冲电源(THU-PEF4)系统,针对小球藻的光合活性和叶绿素质量浓度这2个生物量,重点考察了高压脉冲电场强度、脉冲宽度、脉冲重复频率、电场极性及样品电导率对小球藻处理效果的影响,在此基础上结合双荧光染色法和流式细胞仪研究了高压脉冲电场(PEF)对微藻细胞的穿孔破碎效果。研究发现高压脉冲电场强度和脉冲注入能量密度是影响高压脉冲电场处理效果的关键因素,而脉冲宽度、脉冲重复频率、电场极性对小球藻的处理效果影响不大。当电场强度从2.5 MV/m增加到5.0 MV/m时,20 m S/m电导率下的小球藻细胞破碎率从17.21%增加至83.29%;当脉冲注入能量密度从8.9 k J/L增加到149.52 k J/L时,4.5 MV/m电场强度作用下的小球藻细胞破碎率从9.78%提高到81.78%。Disruption of microalgae cell is an important part of the process of microalgae-based oil extraction. Using Tsinghua self-made high voltage pulsed power supply （THU-PEF4）systems to process Chlorella solutions, we studied how the power supply settings, including the field intensity, width and repetition frequency of high-voltage pulses, electric field polarity, and the sample conductivity, affect the process results, especially from the aspects of the Chlorella photosynthetic activity and Chlorophyll mass concentrations of microalgae. Moreover, using double fluorescent staining and a Flow Cytometry, we investigated the disruption effect of high-voltage pulsed electric field （PEF） on Chlorella cells.Experimental results show that intensity and injected energy density of the applied PEF play the key roles in deter- mining the process effect, however, the pulse width, repetition frequency, and field polarity hardly affect the cell disruption. Increasing the field intensity from 2.5 MV/m to 5.0 MV/m, the cell disruption ratio of the solution, which has conductivity of 20 mS/m,is increased from 17.21% to 83.29%. For the same solution treated by electric field of MV/m, when the injected pulse energy density increases from 8.9 kJ/L to 149.52 kJ/L, its cell disruption ratio will rise from 29.32% to 81.78%.

关 键 词：高压脉冲电场 叶绿素质量浓度 光合活性 细胞破碎 高压脉冲电场强度 脉冲注入能量密度 细胞电穿孔 

分 类 号：Q949.2[生物学—植物学] TE667[石油与天然气工程—油气加工工程]