中和IL－17对博莱霉素诱导的肺纤维化小鼠肺组织Bax／Bcl－2表达的影响  

Effect of Anti-IL-17 on the Expression of Bax/Bcl-2 in Pulmonary Tissues of Mice with Bleomycin-Induced Pulmonary Fibrosis

作　　者：张健[1] 昌军[1] 

机构地区：[1]仙桃市第一人民医院神经内科,湖北省仙桃市433000

出　　处：《医学分子生物学杂志》2016年第4期218-222,共5页Journal of Medical Molecular Biology

摘　　要：目的：研究中和IL－17 A对博莱霉素（ bleomycin， BLM）诱导的肺纤维化小鼠肺组织Bax／Bcl－2表达的影响。方法将小鼠分别分成生理盐水组、 BLM组、 IL－17 A抗体组和IL－17 A抗体对照组，各30只，利用HE染色、 Masson三色胶原纤维染色检测小鼠肺组织纤维化程度，免疫组化方法检测小鼠肺组织Bax／Bcl－2的表达。结果从生理盐水组、到IL－17 A抗体组、再到BLM组和IL－17 A抗体对照组，小鼠肺组织肺泡炎症程度、肺泡壁的增厚情况以及肺泡壁的纤维化程度逐渐加重，差异有显著性意义（P＜0．01）； BLM组和IL－17 A抗体对照组间无明显差异（P＞0．05）。4组间Bax蛋白、 Bcl－2蛋白的表达也逐渐加重，差异均有统计学意义（ P＜0．01和P＜0．05）。 BLM组与抗体对照组间Bax、 Bcl－2的表达均无显著差异（P＞0．05）。结论 BLM可通过IL－17 A来诱导肺纤维化的形成，肺纤维化时肺实质细胞凋亡增多，而中和IL－17 A可通过调节Bax／Bcl－2的表达来抑制肺组织气道上皮的凋亡、达到抑制肺纤维化的目的。Objective To investigate the effect of anti-IL-17 A on the expression of Bax/Bcl-2 in pulmonary tissues of mice with bleomycin （ BLM） -induced pulmonary fibrosis.Methods The mice were divided into saline group, BLM group, anti-LI-17A group and anti-IL-17A control group （ n=30 each） .Hematoxylin-eosin （ HE） staining and Masson’ s Trichrome staining were used to detect the fibrosis of pulmonary tissues in mice and immunohistochemistry to measure the expression of Bax/Bcl-2 in pulmonary tissues.Results The inflammation of the alveoli, the thickening and the fibrosis of the alveolar wall gradually deteriorated from saline group, anti-IL-17 group, BLM group to anti-IL-17 control group, and there were significant differences between groups except be-tween BLM group and anti-IL-17A control group （P〈0.05） .The expression of Bax/Bcl-2 protein was significantly lower in the anti-IL-17 group than in other three groups （ P〈0.01 or P〈0.05 ） .No significant difference was found in the expression of Bax/Bcl-2 protein between BLM group and anti-IL-17 control group （ P〉0.05） .Conclusion BLM can induce the formation of pulmonary fi-brosis, which causes the increase in the apoptosis of pulmonary parenchyma cells.Anti-IL-17 can suppress the apoptosis of the airway epithelial cells and therefore the pulmonary fibrosis by regulating the expression of Bax/Bcl-2.

关 键 词：博莱霉素 肺纤维化 IL-17 A Bax Bcl-2 

分 类 号：R363[医药卫生—病理学]