产7α,15α-双羟基去氢表雄酮的球黑孢菌紫外诱变育种及发酵条件  

Mutantion of Nigrosporasphaericaby ultraviolet mutation to produce 7α,15α-dihydroxyl dehydroepiandrosterone and its fermentation condition

作　　者：唐洁[1] 崔励[1] 吴明杰[1] 

机构地区：[1]大连工业大学轻工与化学工程学院,辽宁大连116034

出　　处：《大连工业大学学报》2016年第4期254-258,共5页Journal of Dalian Polytechnic University

摘　　要：球黑孢菌可以在去氢表雄酮(DHEA)的C-7α和C-15α位进行双羟基化反应,生成7α,15α-双羟基去氢表雄酮(7α,15α-diOH-DHEA)。以球黑孢菌FN-A127为出发菌株进行紫外诱变,筛选出最佳菌株FN-A7,并对FN-A7进行遗传稳定性研究和发酵条件优化。结果表明,紫外诱变最佳照射时间为15min,菌株FN-A7的遗传稳定性良好。在发酵培养基中加入2g/L的DHEA,在8%的接种量、30℃进行发酵,突变菌株FN-A7将DHEA转化为7α,15α-diOH-DHEA的产率可达到31.96%。Nigrospora 15α-diOH-DHEA） by dihydroxy （DHEA）. After ultraviolet mutat stable in 15 min of mutation time inoculation of 8% and DHEA of 2 lation reaction on C-7α and C-15α of dehydroepiandrosterone ion one mutant FN-A7 was obtained. Its genetics characteristic was and the yield of 7α,15α-diOH-DHEA could reach to 31.96% with inoculation of 8% and DHEA of 2 g/L at 30 α.

关 键 词：球黑孢菌 去氢表雄酮 紫外诱变 发酵条件 

分 类 号：Q815[生物学—生物工程]