左乙拉西坦和甘珀酸对癫痫模型大鼠改善作用的比较及相关分子机制研究  

Comparative study of the improving effect of levetiracetam and carbenoxolone on epilepsy model rats and the study of related molecular mechanism

作　　者：贾颐 代晓杰[1] 张莉[1] 刘建军[1] 

机构地区：[1]陕西省西安高新医院神经内科,陕西西安710075

出　　处：《海南医学院学报》2016年第19期2228-2231,2234,共5页Journal of Hainan Medical University

基　　金：陕西省自然科学发展项目(XZ-421G)~~

摘　　要：目的:研究左乙拉西坦和甘珀酸对癫痫模型大鼠癫痫发作情况、神经元凋亡情况以及缝隙连接蛋白表达情况的影响。方法:选择雄性SD大鼠并分为对照组、癫痫模型组、甘珀酸组、左乙拉西坦组,癫痫模型组、甘珀酸组、左乙拉西坦组采用氯化锂-匹罗卡品腹腔注射的方式建立癫痫模型,并分别给予生理盐水、甘珀酸、左乙拉西坦治疗,观察4组大鼠癫痫发作情况并测定大脑海马组织中细胞凋亡情况以及Cx26、Cx30、Cx43、Bcl-2、Bax、Caspase-3的表达量。结果:左乙拉西坦组和甘珀酸组大鼠癫痫发作潜伏期比癫痫模型组显著延长、发作次数显著少于癫痫模型组(P<0.05),左乙拉西坦组大鼠癫痫发作潜伏期比甘珀酸组显著延长、发作次数显著少于甘珀酸组(P<0.05)。左乙拉西坦组和甘珀酸组大鼠海马组织中Cx26、Cx30、Cx43、Bax、Caspase-3的蛋白含量显著低于癫痫模型组(P<0.05),Bcl-2的蛋白含量显著高于癫痫模型组(P<0.05),TUNNEL阳性染色细胞数目显著少于癫痫模型组(P<0.05),Nissl阳性染色细胞数目显著多于癫痫模型组(P<0.05);左乙拉西坦组大鼠海马组织中Cx26、Cx30、Cx43、Bax、Caspase-3的蛋白含量显著低于甘珀酸组(P<0.05),Bcl-2的蛋白含量显著高于甘珀酸组(P<0.05),TUNNEL阳性染色细胞数目显著少于甘珀酸组(P<0.05),Nissl阳性染色细胞数目显著多于甘珀酸组(P<0.05)。结论:左乙拉西坦抑制癫痫模型大鼠癫痫发作的作用优于甘珀酸,抑制缝隙连接蛋白表达以及细胞凋亡是左乙拉西坦发挥抗癫痫作用的分子机制。Objective： To study the effect of levetiracetam and carbenoxolone on epileptic seizures, neuron apoptosis and connexin expression in epilepsy model rats. Methods：Male SD rats were selected and divided into control group, epilepsy model group, carbenoxolone group and levetiracetam group epilepsy model, group, carbenoxolone group and levetiraeetam group re- ceived intraperitoneal injection of lithium chloride--pilocarpine to establish epilepsy models and were given saline, earbenoxolo- ne and levetiracetam treatment respectively. The epileptic seizures of the four groups of rats were observed, and the cell apow tosis as well as Cx26, Cx30, Cx43, Bel-2, Bax and Caspase-3 expression levels in brain hippocampus was determined. Results：Epileptic seizure incubation of levetiracetam group and carbenoxolone group were significantly longer than that of epilepsy mod- el group and the frequency of seizures were significantly less than that of epilepsy model group, epileptic seizure incubation of levetiracetam group was significantly longer than that of carbenoxolone group and the frequency of seizures was significantly less than that of carbenoxolone group. Cx26, Cx30, Cx43, Bax and Caspase-3 protein levels in hippocampus tissue of levetirac- etam group and carbenoxolone group were significantly lower than those of epilepsy model group, Bcl-2 protein levels were sig- nificaatLy higher than that of epilepsy mode!, group, positive TUNNEL staining cell number were significantly 1.ess than that of epilepsy model group, and positive Nissl staining cell number were significantly more than that of epilepsy model group~ Cx26, Cx30, Cx43, Bax and Caspase-3 protein levels in hippocampus tissue of levetiracetam group were significantly lower than those of carbenoxolone group, Bcl-2 protein level was significantly higher than that of carbenoxolone group, positive TUNNEL stai- ning cell number was significantly less than that of carbenoxolone group, and positive Nissl staining cell number was signifi- cantly more than that of carbe

关 键 词：癫痫 左乙拉西坦 缝隙连接蛋白 凋亡 

分 类 号：R742.1[医药卫生—神经病学与精神病学]