片仔癀上调miR-200a抑制大肠癌耐药细胞转移的作用机制  

Mechanism of Pien Tze Huang's effect on suppressing metastasis of large intestine cancer resistant cells via up-regulating mi R-200a

作　　者：沈阿灵[1] 刘丽雅[1] 齐飞[1] 陈宏伟[1] 魏丽慧[1] 林久茂[1] 蔡巧燕[1] 彭军[1] 

机构地区：[1]福建中医药大学中西医结合研究院,福州350122

出　　处：《中华中医药杂志》2016年第9期3682-3686,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81403390)~~

摘　　要：目的:研究片仔癀对大肠癌耐药细胞转移的影响和对mi R-200a及其靶基因E盒结合锌指蛋白ZEB1/2和下游转移相关基因表达的调控作用。方法:MTT法检测不同浓度5-氟尿嘧啶(5-FU)对HCT-8/5-FU细胞和HCT-8细胞活力的影响;采用黏附实验和Transwell法检测细胞黏附、迁移和侵袭能力的改变;通过Q-PCR检测片仔癀对HCT-8/5-FU细胞mi R-200a的表达;采用免疫荧光检测mi R-200a靶基因ZEB1和ZEB2及其下游E-cadherin、N-cadherin的表达。结果:MTT检测结果显示不同浓度的5-FU干预可显著降低HCT-8细胞活力,对HCT-8/5-FU细胞活力影响较小;片仔癀干预显著降低HCT-8/5-FU细胞黏附、迁移和侵袭能力;可显著上调mi R-200a的表达和E-cadherin的表达,下调ZEB1、ZEB2和N-cadherin的表达。结论:片仔癀干预显著抑制大肠癌耐药细胞的转移,且通过调控mi R-200a/ZEB1/2通路及其下游E-cadherin、N-cadhrin的表达可能是其抑制大肠癌转移的重要内在机制。Objective：To evaluate the effects of Pien Tze Huang（PZH） on metastasis of large intestine cancer resistant cells and on regulating the expression of mi R-200 a,its target genes ZEB1/2 and related downstream metastasis genes.Methods：Effects of 5-FU of different concentrations on the viability of HCT-8/5-FU cells and HCT-8 cells were examined by MTT assay.Adhesion assay and Transwell assay were performed to determine the capability of adhesion,migration and invasion of HCT-8/5-FU cells with the treatment of PZH.Q-PCR analysis was used to determine the expression of mi R-200 a of HCT-8/5-FU cells.Immunol uorescence was used to determine the expression of E-cadherin,N-cadherin,ZEB1 and ZEB2.Results：MTT assay showed that the cell viability of HCT-8 cells was significantly decreased with the treatment of 5-FU of different concentrations,while HCT-8/5-FU cells were insensitivity to 5-FU treatment.PZH treatment greatly inhibited the capability of adhesion,migration and invasion of HCT-8/5-FU cells.Furthermore,it showed that PZH treatment up-regulated the expression of mi R-200 a and E-cadherin and down-regulated the expression of ZEB1,ZEB2 and N-cadherin.Conclusion：PZH can significantly inhibit metastasis of large intestine cancer resistant cells which might be important mechanism according to regulating the pathway of mi R-200a/ZEB1/2 and the expression of their downstream genes,E-cadherin and N-cadherin.

关 键 词：大肠癌 片仔癀 转移 耐药 MI RNA MI R-200a 

分 类 号：R735.34[医药卫生—肿瘤]