欧美杨108高效组培再生系统  

Effective tissue culture system for Populus euramericana ‘Guariento'

作　　者：周洲[1] 李永丽[1] 安世恒[2] 胡建军[3] 

机构地区：[1]河南科技大学林学院,河南洛阳471003 [2]河南农业大学植保学院,河南郑州450002 [3]中国林业科学研究院林业研究所,北京100091

出　　处：《中南林业科技大学学报》2016年第7期1-6,共6页Journal of Central South University of Forestry & Technology

基　　金：国家自然科学基金项目(U1204324);河南省科技攻关重点项目(132102110037)

摘　　要：欧美杨108生产性状优良,是基因工程转化的理想受体,高效的组培再生系统是转化工作的基础。以欧美杨108茎段、叶柄、叶片3种外植体为起始材料,确定了植株再生过程中诱导丛生芽、芽的茎伸长和生根培养的最优培养条件,建立了稳定高效的再生培养系统。结果表明:基本培养基MS优于WPM;最适从生芽诱导培养基为MS+TDZ 0.05 mg/L+6-BA 0.05 mg/L+NAA 0.05 mg/L+蔗糖30 g/L,茎段、叶柄、叶片3种外植体丛生芽诱导率分别达100%、100%和86%。丛生芽需要进行茎伸长诱导的环节,茎伸长诱导效率最高是MS+KT 0.5 mg/L+GA3 2 mg/L+果糖30 g/L培养基,茎段和叶柄丛生芽茎伸长诱导最好,诱导培养20 d,得到平均芽长3 cm的有效芽,增殖系数大于4。抽茎芽转入生根培养前,须经MS基本培养基壮化培养。在1/2 MS+IAA0.02 mg/L+IBA 0.02 mg/L+AC3 g/L+蔗糖30 g/L培养基上,试管苗生根率可达100%,苗体健壮。欧美杨108高效组培再生系统为其遗传转化奠定了基础。Production behaviors of P. euramericana‘Guariento’ show that it is a superior variety, so it is a good recipient material for gene transformation. Stem, petiole and leaf were cultured as the initiation explants developing from tender branches outdoor to investigate the micropropagation. The key factors affecting the growth of P. euramericana‘Guariento’ in vitro and the optimal tissue culture conditions for it were ascertained. The optimum basic culture medium was MS. Cultured explants on MS＋TDZ 0.05 mg/L＋6-BA 0.05 mg/L＋NAA 0.05 mg/L＋Sucrose 30 g/L was the best way of inducement and differentiation, with inducement rate 100%, 100%and 86%for stem, petiole and leaf from tissue culture plantlets. For elongation of the induced buds, the suitable culture medium was MS＋KT 0.5 mg/L＋GA3 2 mg/L＋Fructose 30 g/L. More than 4 shoots about 3 cm high were elongated from the induced buds after 20 days. The optimal subculture medium and root inducement culture medium for shoot were 1/2 MS＋IAA 0.02 mg/L＋IBA 0.02 mg/L＋AC3 g/L＋Sucrose 30 g/L, and the rooting rate was 100%. The high frequency regeneration system for P.euramericana‘Guariento’ in vitro provides a basis for gene modiifcation in this study.

关 键 词：欧美杨108 离体培养 芽伸长诱导 芽壮化培养 

分 类 号：S772.3[农业科学—森林工程]