土槿皮乙酸诱导脑胶质瘤细胞株U87阻滞于M期并发生凋亡  

Pseudolaric acid B induces glioblastoma cell line U87 mitotic arrest and apoptosis

作　　者：何露[1,2] 温创宇[3] 王慧慧[3] 杨湘玲[3] 刘焕亮[4,3] 李中[1,2,5] 

机构地区：[1]中山大学附属第六医院神经科,广东广州510655 [2]中山大学中山医学院广东省脑功能与脑疾病重点实验室,广东广州510080 [3]中山大学附属第六医院广东省胃肠病学研究所,广东广州510655 [4]中山大学附属第六医院检验科,广东广州510655 [5]中山大学深圳研究院,广东深圳518057

出　　处：《中国病理生理杂志》2016年第9期1574-1578,共5页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目(No.2015A030313128);广东省自然科学基金资助项目(No.2014A030307025);广州市天河区科技计划重点项目(No.201404KW028);深圳市科技计划项目(No.JCYJ20150403151851068)

摘　　要：目的:通过研究土槿皮乙酸对脑胶质瘤细胞株U87增殖与凋亡的影响,探讨土槿皮乙酸对脑胶质瘤的潜在应用价值。方法:用不同浓度土槿皮乙酸处理U87细胞,并于不同时点观察细胞形态改变;采用MTS法对U87细胞进行细胞活力测定;采用流式细胞术和Western blot法检测土槿皮乙酸对细胞周期的影响;采用Annexin V/PI双染流式细胞术检测细胞凋亡;利用Western blot法检测caspase通路相关蛋白(cleaved PARP、caspase-3、procaspase-9和caspase-8)的变化情况。结果:土槿皮乙酸明显抑制脑胶质瘤U87细胞的活力,能使细胞阻滞于M期,并通过caspase通路诱导细胞发生凋亡。结论:土槿皮乙酸能使脑胶质瘤细胞株U87细胞阻滞于M期,并诱导其凋亡。AIM： To investigate the effects of pseudolaric acid B on the growth and apoptosis of glioblastoma cell line U87. METHODS： The cell morphological changes were observed under inverted microscope. The cell viability was evaluated by MTS assay. The cell cycle was analyzed by flow cytometry and Western blot. The cell apoptosis was detected by flow cytometry. The changes of apoptosis-related proteins cleaved PARP,caspase-3,procaspase-9 and caspase-8were determined by Western blot. RESULTS： Pseudolaric acid B inhibited the viability of U87 cells,arrested U87 cells in mitosis. Apoptosis of U87 cells was induced by pseudolaric acid B. The caspase pathway was activated. CONCLUSION：Pseudolaric acid B induces glioblastoma cell line U87 mitotic arrest and apoptosis.

关 键 词：脑胶质瘤 土槿皮乙酸 细胞周期 细胞活力 细胞凋亡 

分 类 号：R739.4[医药卫生—肿瘤] R363[医药卫生—临床医学]